Abstract：Extracellular matrix (ECM) hydrogels derived from natural tissues and organs are generally removing cellular components while leaving the native ECM composition and structure via combining physical, chemical and enzymatic approaches to obtain polymeric biomaterials that possess the capability for creating a superior microenvironment for cell adhesion, proliferation and differentiation. Recently, the application of ECM hydrogel for tissue engineering and regenerative medicine has gained increasing attention due to their excellent biocompatibility, biodegradability and tissue regeneration ability. The present review firstly introduced the basic characteristics and material properties of these ECM hydrogels, the content included their internal composition and structure, tissue specificity and potential immune rejection. Following contents focused on the application of the hydrogels in tissue engineering from three aspects, including the cell culture investigation, preclinical research and clinical translation in disease models. Finally, further advantages of utilizing ECM hydrogels on tissue regeneration and their shortcomings were discussed. In conclusion, as a naturally derived proteinaceous biomaterial, ECM hydrogel holds a bright prospect for constructing engineered tissue and repairing tissue defects.
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