Abstract：The decellularized blood vessel prepared by decellularizing the natural blood vessel is considered as one important kind of tissue engineering vascular scaffolds and has broad application prospects. However, the preparation of extracellular matrix (ECM) still lacks uniform standards. The choice of decellularization methods depends on the tissue source and the application of the ECM. The decellularization protocols used for preparing ECM are very important, especially for the ECM scaffolds such as decellularized blood vessels that need to withstand the blood press for a long time. The maintenance of ECM integrity and cell removal efficiency are highly dependent on the specific approaches used for decellularization. This article reviewed decellularization methods applied to blood vessels, including physical, chemical, enzymatic and serum applications in the preparation of decellularized blood vessels. The decellularization efficiency of different decellularization methods and their effects on the tissue structure, mechanical properties and biological activity of the vascular matrix were compared, and the importance of developing reasonable decellularization protocols was emphasized.
成津, 王聪, 谷涌泉. 脱细胞技术在组织工程血管中的应用进展[J]. 中国生物医学工程学报, 2021, 40(1): 118-123.
Cheng Jin, Wang Cong, Gu Yongquan. Progress on the Application of Decellularization Techniques in Tissue Engineering Vascular Scaffolds. Chinese Journal of Biomedical Engineering, 2021, 40(1): 118-123.
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