Progress in Application of Hydrogels and Mesenchymal Stem Cells in Tissue Engineering
Li Min1, Meng Xiangjing1#*, Zhang Xiangkui1, Liu Bo1, Duan Chonggang1, Zhang Lanying1, Zhang Daizhou1, Ling Peixue1, 2
1 Shandong Provincial Key Laboratory of Biopharmaceuticals, Shandong Academy of Pharmaceutical Sciences, Jinan 250101, China; 2 Shandong Provincial Key Laboratory of Mucosal and Transdermal Drug Delivery Technologies, Shandong Freda Pharmaceutical Group Co., Ltd., Jinan 250101, China
Abstract：With the development of tissue engineering, there is an increasing focus on using hydrogel as scaffolds and 3D culture modes for tissue and organ regeneration. Hydrogel is formed by hydrophilic polymer, copolymer or a monomer macromolecule capable of forming a macromolecular chain, which can absorb a large amount of water and maintain a three-dimensional structure. Because of its good biocompatibility, entrapment of cells and bioactive molecules and effective delivery, they are widely used in drug delivery, tissue engineering and so on in the field of biomedicine. Mesenchymal stem cells can be obtained in bone marrow, fat, umbilical cord and other tissues with low immunogenicity and multidirectional differentiation potential, which are the preferred cells for 3D cultures and cell therapies. At present, the culture mode of mesenchymal stem cells is mainly 2D. The 2D culture mode of mesenchymal stem cells leads to low reproduction rate and cannot simulate the growth environment in vivo. Hydrogel materials as scaffolds of 3D culture have good compatibility, can simulate the growth environment in vivo, and have great potential in repairing damaged cartilage, such as bone, skin and heart tissues. In this review, we summarized the applications of hydrogels and mesenchymal stem cells in tissue engineering. We showed the development of hydrogel materials and 3D culture mode of mesenchymal stem cells in different tissues, showing that the 3D culture mode of hydrogel materials and mesenchymal stem cells makes it possible to regenerate and repair tissues and organs, and providing a reference for the further study of the application of hydrogel and stem cell.
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