Abstract：With the rapid development of tissue engineering, scaffolds with 3D structures have become one of the key factors for the treatment of the spinal cord injury. In this work the PLGA porous scaffold fabricated with low temperature deposition manufacturing (LDM) technique was tested in rehabilitation of spinal cord injury. In the paper, poly (lactic-co-glycolic acid, PLGA) was selected to fabricate the spinal cord scaffold through LDM technique. The regeneration of spinal cord injury was induced by implanting the PLGA scaffold containing SCs into the transected spinal cords of Sprague Dawley (SD) rats (total 30). The PLGA scaffold was characterized as good porosity of 87.64%, hydrophilicity and considerable biodegradability. The degradation rate showed the trend of slow at the beginning and getting faster at the later period. In the experiment, the scaffolds were steady in the first 16 weeks, and the degradation rate began to be accelerated from the 20th week. In addition, the effect of PLGA scaffold on the cell proliferation and cytotoxicity was evaluated by culturing Schwann cells (SCs) on the surface of the scaffold, showing that SCs spread and grew well on the PLGA scaffold with good proliferation. The BBB score indicated that paraplegia of the rats improved from the 4th week, and slightly movement was visible on the posterior limbs of the injured rates. The investigation of section staining showed that the recovery of the spinal cord was considerably improved after implanting with the PLGA scaffold, which indicated that the PLGA scaffold exhibited great potential to be applied in the spinal cord injury. The PLGA porous scaffold fabricated with LDM has material effect in rehabilitation of spinal cord injury.
史廷春, 周 陶, 孙芳芳, 王瑞艳, 安 晓. 脊髓损伤的PLGA低温成形组织工程支架修复[J]. 中国生物医学工程学报, 2017, 36(1): 83-91.
Shi Tingchun, Zhou Tao, Sun Fangfang, Wang Ruiyan, An Xiao. Low Temperature Forming Tissue Engineering PLGA Scaffold to Repair Spinal Cord Injury in Rats. Chinese Journal of Biomedical Engineering, 2017, 36(1): 83-91.
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