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中国生物医学工程学报  2023, Vol. 42 Issue (2): 189-200    DOI: 10.3969/j.issn.0258-8021.2023.02.007
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基于散射OCT量化表征组织工程中细胞活性分布的研究
卢明1, 王玲1,2*, 杨珊珊1,2, 徐铭恩1,2
1(杭州电子科技大学自动化学院,杭州 310018)
2(浙江省医学信息与生物三维打印重点实验室,杭州 310018)
Characterization of Cell Activity Distribution in Tissue Engineering Based on Scattering OCT
Lu Ming1, Wang Ling1,2*, Yang Shanshan1,2, Xu Mingen1,2
1(School of Automation, Hangzhou Dianzi University, Hangzhou 310018, China)
2(Zhejiang Provincial Key Lab of Medical Information and Three-Dimensional Bio-Printing,Hangzhou 310018, China)
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摘要 非侵入且无需标记的细胞活性分布检测在组织工程中具有重要意义。本研究利用散射光学相干层析成像(OCT)技术量化表征三维组织模型中的细胞活性分布,提出优化的深度解析(ODR)散射算法以重建细胞/材料的散射对比增强图像,定量分析散射系数与细胞浓度、存活状态的关联性,表征三维组织内的细胞活性分布。多层样本模型实验结果表明,采用ODR散射算法能够提高样本散射成像的灵敏度和深度;人肝癌细胞C3A和人类真皮成纤维细胞负载的水凝胶实验验证了细胞浓度、细胞活性均和散射系数线性相关,皮尔逊相关系数分别为0.920 7和0.991 3,表明可用散射系数量化表征水凝胶支架中的细胞浓度和细胞存活状态。基于ODR的散射OCT可以无损无标记检测组织工程支架中的细胞活性分布,可对载细胞支架的细胞活性分布开展长期研究,有望成为疾病模型构建、药物筛选和细胞治疗的监测工具。
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卢明
王玲
杨珊珊
徐铭恩
关键词 光学相干层析成像散射系数组织工程细胞活性分布水凝胶    
Abstract:Non-invasive and label-free detection of cell activity distribution in tissue engineering is of great significance. This paper proposed a scattering optical coherence tomography (OCT) technology to quantify and characterizing the cell activity distribution in a three-dimensional tissue model, and proposed an optimized depth-resolved (ODR) scattering algorithm to reconstruct the cell/material scatter contrast enhanced images, achieving the quantitative analysis of the correlation between scattering coefficients, cell concentration and activity status, and characterize the cell activity distribution in three-dimensional tissues. The experimental results of multi-layer sample model showed that the ODR scattering algorithm was able to improve the sensitivity and depth of sample scattering imaging. The human hepatocellular carcinoma cell C3A and human dermal fibroblast cell-laden hydrogel were used to verify that the cell concentration and cell activity in the material are linearly correlated with the scattering coefficient (92.07%) and Pearson correlation coefficient (99.13%) respectively, and the scattering coefficient could be used to quantify the cell concentration and survival status in the hydrogel scaffold. ODR-based scattering OCT realized the non-invasive and label-free detect of cell activity distribution in tissue engineering scaffolds, allowing to carry out long-term research on the cell activity distribution of cell-laden scaffolds, therefore, having potentials of a powerful monitoring tool for disease model construction, drug screening and cell therapy.
Key wordsoptical coherence tomography    scattering coefficient    tissue engineering    cell activity distribution    hydrogel
收稿日期: 2021-11-08     
PACS:  R318  
基金资助:国家自然科学基金重点项目(31927801);浙江省重点研发计划项目(2022C01123)
通讯作者: *E-mail: lingw@hdu.edu.cn   
引用本文:   
卢明, 王玲, 杨珊珊, 徐铭恩. 基于散射OCT量化表征组织工程中细胞活性分布的研究[J]. 中国生物医学工程学报, 2023, 42(2): 189-200.
Lu Ming, Wang Ling, Yang Shanshan, Xu Mingen. Characterization of Cell Activity Distribution in Tissue Engineering Based on Scattering OCT. Chinese Journal of Biomedical Engineering, 2023, 42(2): 189-200.
链接本文:  
http://cjbme.csbme.org/CN/10.3969/j.issn.0258-8021.2023.02.007     或     http://cjbme.csbme.org/CN/Y2023/V42/I2/189
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