Effects of Surface Nano-Pattern Modification of Regenerated Silk Fibroin Film on CellAdhesion and Proliferation
Ouyang Qinjun, Liu Xiaojiao, Yao Xiang*, Zhang Yaopeng
(College of Materials Science and Engineering, State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, China)
Abstract：Improving the cytocompatibility of regenerated silk fibroin (RSF) biomaterials is very important to expand its application in the biomedical fields of cell culture and tissue repair. This study prepared three kinds of special nano-island-patterned RSF films by plasma etching with different etching times, and further investigated their effects on the adhesion and proliferation of NIH3T3 cells, and all quantification experiments were set at least three replicates (n≥3). The micrograph observation results after 1 d of cell culture showed that cells on the nano-pattern modified RSF films had better adhesion morphology when compared to those on the non-modified flat RSF film. The results of OD value detected by CCK-8 after 1 and 4 d of cell culture showed that nano-pattern modified RSF films significantly promoted the cell proliferation compared to the non-modified flat RSF film. Moreover, cells on the 30 min-etched film presented the highest OD value (OD=1.13±0.32), which was much higher than that of on the non-modified flat RSF film (OD=0.46±0.03, P＜0.001). In summary, plasma etching provided a simple, rapid, and large-scale nano-pattern modification strategy to improve the cytocompatibility of RSF materials, which is expected to provide important references and guidance for the surface modification of RSF and other biomaterials.
欧阳秦君, 刘晓娇, 姚响, 张耀鹏. 再生丝素蛋白膜表面的纳米图案化修饰对细胞黏附和增殖的影响[J]. 中国生物医学工程学报, 2023, 42(2): 201-209.
Ouyang Qinjun, Liu Xiaojiao, Yao Xiang, Zhang Yaopeng. Effects of Surface Nano-Pattern Modification of Regenerated Silk Fibroin Film on CellAdhesion and Proliferation. Chinese Journal of Biomedical Engineering, 2023, 42(2): 201-209.
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