1(Department of Anesthesiology, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100730, China) 2(Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China)
Abstract：The effects of hydrogel dressings on the immune cells and vascular endothelial cells are closely related to wound healing. This work is aimed to investigate the effects of a carbon fiber reinforced polyvinyl alcohol conductive hydrogel (PVA/CFs) on inflammatory response in mouse macrophages and the formation of adherent junctions between HUVEC cells. The above two kinds of cells were cultured on PVA and PVA/CFs with CF content of 1.5%, 3% and 5%, respectively. The expression of TNF-α, IL-1β and IL-6 in inflammatory response genes of macrophages (RAW264.7) was detected by real-time PCR. The cytokins secretion of TNF-α, IL-1β and IL-6 were tested by ELISA kits. The growth and proliferation of human umbilical vein endothelial cells (HUVEC) and RAW264.7 on PVA/CFs were detected by CCK-8 assay. The protein expression of VE-Cadherin in HUVEC was determined by laser scanning confocal microscopy and western blot assay. All quantitative detection experiments were set at least three replicates (n≥3). PVA/CFs did not significantly change the gene expression levels of TNF-α, IL-1β and IL-6 and the cytokins secretion levels in RAW264.7 cells compared with PVA. All groups were no significant difference (P>0.05). However, the secretion of TNF-α, IL-1β and IL-6 in RAW264.7 cells treated with LPS was increased by 2.35, 4.27 and 27.1 folds of that in the control group, respectively. The expression of VE-cadherin in HUVEC cells on PVA/CFs was increased, suggesting that PVA/CFs could strengthen adherent junctions of endothelial cells. In addition, PVA/CFs could support the growth and proliferation of RAW264.7 and HUVEC cells, and there was no significant difference in cell viability between PVA/CFs and PVA groups (P>0.05). PVA/CFs not only did not cause any severe inflammatory responses of macrophages, but also supported the growth and proliferation of macrophages and endothelial cells, and promoted the formation of integrated adherent junctions between endothelial cells.
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