Abstract：The aim of this study is to investigate the effect of the composite liquid dressing in the treatment of acute skin ulcer for further applications in the prevention of surgical wound infection and promotion of wound healing. The composite liquid dressing (CLD) was prepared by carboxymethyl chitosan (CMC), polyvinyl butyral (PVB) and ethanol solution, according to the certain formulation. The performance and biological safety of CLD were evaluated by experiments of waterproof, breathable, bacteria resistance, and cell toxicity. Forty healthy adult SD rats, half male and half female, were made wound, carboxymethyl chitosan meanwhile different concentration at 1.0 mg/mL, 10.0 mg/mL, 30.0 mg/mLwas applied to the wound. The therapeutic effect of the wound composite liquid dressing was studied by daily observation and HE staining. The upper membrane liquid of CLD between 1.8～ 2.3 mm had very good waterproof permeability and resistance to bacteria. In animal models, the wound healing rate of the experimental group (10.0 and 30.0 mg/mL CMC) was 65.42% and 67.38%, which was higher than that of control group with significant difference (P< 0.01) on the 7th day. The wound healing rate of the experimental group (10.0 and 30.0 mg/mL CMC) was 100% on the 14th day. Seven days after the operations, the wound healing rate of the experimental group (10.0 and 30.0 mg/mL CMC), the stratified squamous epithelium that formed the epidermis and the dermis consisted of thin collagen fibers. The organization began to fall into the interior; the normal dermis with a thick, coarse collagen fiber was connected to a thin collagen fiber. The stratified squamous epithelium of the epidermis was much larger than that of the control group from 3 to 4. The wound was connected with dermal connective tissue. The skin was very close to the normal skin on the 12th day. The results showed that the dressing functioned (10.0 mg/mL CMC ) as a good barrier against the penetration of infection, it was waterproof, had a suitable water vapor transmission rate, good biosafety and a rapid wound closure rate.
[1] Madaghiele M, Demitri C, Sannino A, et al. Polymeric hydrogels for burn wound care: Advanced skin wound dressings and regenerative templates[J]. Burns and Trauma, 2014, 2(4): 153-161.
[2] Naguib G, Al-Mashat H, Desta T, et al. Diabetes prolongs the inflammatory response to a bacterial stimulus through cytokine dysregulation[J]. Journal of Investigative Dermatology, 2004, 123(1): 87-92.
[3] Goren I, Müller E, Pfeilschifter J, et al. Severely impaired insulin signaling in chronic wounds of diabetic ob/ob mice: a potential role of tumor necrosis factor-α[J]. The American Journal of Pathology, 2006, 168(3): 765-777.
[4] Luo Yangchao, Teng Zi, Chen Pei, et al. Encapsulation of indole-3-carbinol and 3, 3'-diindolylmethane in zein/carboxymethyl chitosan nanoparticles withcontrolled release property and improved stability[J]. Food Chemistry, 2013, 139(1): 224-230.
[5] 王文庆, 侯丽, 吴平, 等. 接触性创面敷料阻菌性试验方法标准介绍及改进构想[J]. 中国医疗器械信息, 2010, 16(7): 40-41.
[6] Borghi AA, Oliveiranascimento L, Stephano MA, et al. Cytotoxicity of doxycycline effluent generated by the fenton process[J]. The Scientific World Journal, 2013,2014:439461-439461.
[7] Queen D, Gaylor JDS, Evans JH, et al. The preclinical evaluation of the water vapour transmission rate through burn wound dressings[J]. Biomaterials, 1987, 8(5): 367-371.
[8] 刘德伍, 刘德明. 组织工程化人工皮肤的构建与应用[J]. 中国临床康复, 2002, 6(10): 1421-1422.
[9] Rasuli S, Lotfi M, Seyedamini B, et al. The effects of biological, synthetic and traditional dressing on pain intensity of burn wound in children[J]. Iran Journal of Nursing, 2013, 26(85): 15-25.
[10] Winter GD. Formation of the scab and the rate of epithelization of superficial wounds in the skin of the young domestic pig[J]. 1995,4(8):368-371.
[11] Qu Meijun, Wang Weiqing. Efficacy observation of dermlin for skin injury of the neck in throat cancer patients accepted radiotherapy[J]. China Pharmacy, 2013, 18: 028.
[12] Wei Wei, Wang Yumei. Effect of dermlin wound healing enhancer (paste) on persistent amiodarone therapy induced phlebitis[J]. Journal of Nursing Science, 2012, 20: 046.
[13] Wang Lingchong, Chen Xiguang, Xu Chenquan, et al. Plasma protein adsorption pattern and tissue-implant reaction of poly(vinylalcohol)/carboxymethyl-chitosan blend films[J]. Journal of Biomaterials Science, Polymer Edition, 2008, 19(1): 113-129.
[14] Ho YC, Wu SJ, Mi FL, et al. Thiol-modified chitosan sulfate nanoparticles for protection and release of basic fibroblast growth factor[J]. Bioconjugate chemistry, 2009, 21(1): 28-38.
[15] Liang Xiaofei, Tian Hui, Luo Hao, et al. Novel quaternized chitosan and polymeric micelles with cross-linked ionic cores for prolonged release of minocycline[J]. Journal of Biomaterials Science, Polymer Edition, 2009, 20(1): 115-131.
[16] Wang Gan, Lu Guangyuan, Ao Qiang, et al. Preparation of cross-linked carboxymethyl chitosan for repairing sciatic nerve injury in rats[J]. Biotechnology Lsetters, 2010, 32(1): 59-66.
[17] 吴伟萍, 彭承宏, 韩宝三, 等. 羧甲基壳聚糖在大鼠创伤出血模型的止血效果的观察[J]. 外科理论与实践, 2010, 15(3): 240-244.
