C6位氧化型细菌纤维素/壳聚糖复合材料的制备及表征

doi:10.3969/j.issn.0258-8021. 2014. 05.011

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摘要细菌纤维素是天然的骨组织工程支架材料。为了改善其降解性并使其微观结构具有可调控性，利用2,2,6,6-四甲基-1-哌啶酮(TEMPO)/NaBr/NaClO体系，对细菌纤维素C6位羟基进行选择性催化氧化，氧化后的细菌纤维素在活化剂1-乙基-3-(3-二甲基氨丙基)-碳化二亚胺(EDC)/N-羟基琥珀酰亚胺(NHS)存在的条件下与壳聚糖发生交联。将交联产物装入圆柱形模具，经冷冻干燥即得氧化型细菌纤维素/壳聚糖复合材料支架。通过FTIR、13CNMR、XRD、体外降解率测定、孔隙率测定和场发射扫描电镜(FESEM)对复合材料支架进行表征。
FTIR和13CNMR结果表明氧化型细菌纤维素与壳聚糖成功发生交联；XRD交联产物的结晶度随着壳聚糖用量的增大而减小，但减小的幅度不大(≤31%)；体外降解率测定表明氧化产物和复合产物的降解性均明显增大，其中当((OBC—COOH)/(CH—NH2))/(n/n)＝1/45时，复合支架在8周内的降解率达402%；FESEM和孔隙率测定结果表明，OBCCH支架的孔径和孔隙率
大于BC和OBC，当((OBC—COOH)/(CH—NH\-2))/(n/n)＝1/45时，复合支架的孔隙率最大，为97%。实验结果表明，OBCCH是一种理想的具有良好降解性和微观结构可调控性的骨组织工程支架材料。

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	廖世波¹
	2黄淑玉² 赖琛¹奚廷斐¹

关键词 ：细菌纤维素, 2,2,6,6-四甲基-1-哌啶酮, 壳聚糖, 复合材料

Abstract：Bacterial cellulose (BC) is a natural scaffold for bone tissue engineering. To improve its degradability and microstructural controllability, C6oxidated bacterial cellulose/chitosan (OBC/CH) composite scaffolds were prepared by lyophilization of a mixture of OBC suspension and CH solution in a cylindrical mold. The original BC was subjected to 2,2,6,6tetramethylpyperidine1oxyl radical (TEMPO)/NaBr/NaClOmediated oxidation to introduce surface carboxyl groups before mixing. The integration of CH within OBC was performed by 1ethyl3(3dimethylaminopropyl) carbodiimide/Nhydroxysuccinimide (EDC/NHS)mediated crosslinking. The products were characterized by FTIR, solidstate 13CNMR, XRD, in vitro degradation, porosity and FESEM. The FTIR and 13CNMR results show that OBC and CH were crosslinked successfully; XRD result shows that the crystallinity of the products was slightly decreased with the increase of CH content, the difference was less than 31%; the degradability of OBC and OBCCH in vitro was increased significantly, and the degradation rate within 8 weeks was 402% when ((OBCCOOH)/(CHNH2))/(n/n)＝1/45; FESEM and porosity results show that the pore size and porosity of OBCCH scaffolds were greater than that of BC and OBC scaffolds, and the maximum porosity was reached 97% when ((OBCCOOH)/(CH0NH2))/(n/n)＝1/45. In conclusion, OBCCH is an ideal scaffold for bone tissue engineering, with good degradability and microstructural controllability.

Key words： bacterial cellulose TEMPO chitosan composite

基金资助:深圳市知识创新计划项目（JCYJ20130402164725011）；国家重大科学研究计划项目（2012CB933603）

引用本文:

廖世波^1,2黄淑玉² 赖琛¹奚廷斐¹LIAO ShiBo^1,2HUANG Shu Yu²LAI Chen¹XI TingFei^1#*WU Min^2LIU Yu Ping²YAN Yi Min². Preparation and Characterization of C6Oxidated Bacterial Cellulose/Chitosan Composite. journal1, 2014, 33(5): 600-608.

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http://cjbme.csbme.org/CN/ 10.3969/j.issn.0258-8021. 2014. 05.011 或 http://cjbme.csbme.org/CN/Y2014/V33/I5/600

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