仿生嗅觉与味觉传感技术及其应用的研究进展

doi:10.3969/j.issn.0258-8021. 2014. 05.012

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摘要动物的化学感受系统具有惊人的能力，能够在极低的浓度下识别数千种分子结构各异的化学物质，其灵敏性、选择性和响应速度都远高于当前的人工识别系统。近年来，研究者致力于利用完善的天然分子识别机制，开发出一种与传统嗅觉/味觉传感器相比更具仿生意义的新型化学探测系统，即仿生嗅觉/味觉传感器。仿生嗅觉/味觉传感器通常将动物嗅觉味觉的受体、细胞和组织作为敏感材料，然后使用多种人工检测方法，如光学、电化学和声波检测器件等，实现对化学物质的检测和识别。文中概述了动物嗅觉/味觉感受系统对化学物质的信息处理机制，并结合本实验室的工作，综述了目前仿生嗅觉/味觉传感器的常用技术及其未来发展趋势，并展望了其在医疗、工业和军事等领域的应用前景。

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关键词 ：仿生, 嗅觉, 味觉, 生物传感, 化学传感

Abstract：The biological chemical sensory system has the ability to discriminate and identify thousands of chemical compounds at very low concentrations with extremely higher sensitivity and specificity than current artificial sensing devices. Recently, many researchers are trying to develop novel chemical detecting systems called as bioinspired olfaction/gustation sensors which take advantages of optimized natural molecular recognition. Bioinspired olfaction/gustation sensors consist of a primary transducer and a secondary transducer. The primary transducer is the biological recognition element of molecules such as biological tissues, cells and proteins. The second ary transducers such as optical devices, electrochemistry devices and acousticwave devices are the biochips for the detection of binding of chemical compounds to the primary transducers. In this article, we introduced the mechanism of biological sense of smell and taste, and outlined the latest advances of bioinspired olfaction/gustation sensors. Current research trends and future challenges in this field will be discussed.
〖KH2D〗
〖WTHZ〗Key words：〖WTBZ〗〖BP(〗┣┣（英）关键词┫┫〖BP)〗

Key words： bioinspiring olfaction gustation biosensor chemical sense

基金资助:国家自然科学基金重大国际合作项目（61320106002）；海外学者合作基金（31228008）；高校博士点基金（20120101130011）
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引用本文:

秦臻¹董琪¹胡靓^1，2HUANG Li Quan^{2QIN Zhen¹DONG Qi HU Liang HUANG LiQuan ²HSIA Jimmy³WANG Ping^1#*. Recent Advances in Bioinspired Olfaction/Gustation Sensors and their Applications#br#. journal1, 2014, 33(5): 609-619.}

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http://cjbme.csbme.org/CN/10.3969/j.issn.0258-8021. 2014. 05.012 或 http://cjbme.csbme.org/CN/Y2014/V33/I5/609

［1］Stephan A, Bücking M, Steinhart H. Novel analytical tools for food flavours［J］. Food Research International, 2000, 33(3): 199-209.
［2］Persaud K, Dodd G. Analysis of discrimination mechanisms in the mammalian olfactory system using a model nose［J］. Nature,1982,299(5881):352-355. 
［3］Toko K. Taste sensor ［J］. Sensors and Actuators B: Chemical, 2000, 64(1): 205-215.
［4］李光, 傅均, 张佳. 基于嗅觉模型的电子鼻仿生信息处理技术研究进展［J］. 科学通报, 2008, 53(22): 2674-2686.
［5］Gutierrez-Osuna R. Pattern analysis for machine olfaction: a review ［J］. IEEE Sensors Journal, 2002, 2(3): 189-202.
［6］Gpel W, Ziegler C, Breer H, et al. Bioelectronic noses: a status report part I ［J］. Biosensors and Bioelectronics, 1998, 13(3): 479-493.
［7］Firestein S. How the olfactory system makes sense of scents ［J］. Nature, 2001, 413(6852): 211-218.
［8］刘清君. 神经芯片及其在生物嗅觉传感机理中的研究［D］. 杭州：浙江大学, 2006.
［9］Boekhoff I, Tareilus E, Strotmann J, et al. Rapid activation of alternative second messenger pathways in olfactory cilia from rats by different odorants［J］. The EMBO journal, 1990, 9(8): 2453-2458. 
［10］Malnic B, Godfrey PA, Buck LB. The human olfactory receptor gene family ［J］. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(8): 2584-2589.
［11］Touhara K. Odor discrimination by G proteincoupled olfactory receptors ［J］. Microscopy Research and Technique, 2002, 58(3): 135-141.
［12］谭洁, 罗敏敏. 嗅球对嗅觉信息的处理［J］. 生物物理学报, 2010, 26(3): 194-208.
［13］Wood I K. Neuroscience: Exploring the brain［J］. Journal of Child and Family Studies, 1996, 5(3): 377-379.
［14］DeFazio RA, Dvoryanchikov G, Maruyama Y, et al. Separate populations of receptor cells and presynaptic cells in mouse taste buds ［J］. The Journal of neuroscience, 2006, 26(15): 3971-3980.
［15］Matsunami H, Montmayeur JP, Buck LB. A family of candidate taste receptors in human and mouse［J］. Nature, 2000, 404(6778): 601-604.
［16］Chandrashekar J, Hoon MA, Ryba NJP, et al. The receptors and cells for mammalian taste［J］. Nature, 2006, 444(7117): 288-294.
［17］Gilbertson TA, Boughter JD, Zhang H, et al. Distribution of gustatory sensitivities in rat taste cells: wholecell responses to apical chemical stimulation［J］. The Journal of Neuroscience, 2001, 21(13): 4931-4941.
［18］Chandrashekar J, Hoon MA, Ryba NJP, et al. The receptors and cells for mammalian taste ［J］. Nature, 2006, 444(7117): 288-294.
［19］Ishimaru Y. Molecular mechanisms of taste transduction in vertebrates［J］. Odontology, 2009, 97(1): 1-7.
［20］Vosshall LB, Stocker RF. Molecular architecture of smell and taste in Drosophila［J］. Annu Rev Neurosci, 2007, 30: 505-533.
［21］Schütz S, Schning MJ, Schroth P, et al. An insectbased BioFET as a bioelectronic nose［J］. Sensors and Actuators B: Chemical, 2000, 65(1): 291-295.
［22］Huotari MJ. Biosensing by insect olfactory receptor neurons ［J］. Sensors and Actuators B: Chemical, 2000, 71(3): 212-222.
［23］Liu Q, Cai H, Xu Y, et al. Olfactory cellbased biosensor: a first step towards a neurochip of bioelectronic nose ［J］. Biosensors and Bioelectronics, 2006, 22(2): 318-322.［24］Liu Q, Ye W, Xiao L, et al. Extracellular potentials recording in intact olfactory epithelium by microelectrode array for a bioelectronic nose［J］. Biosensors and Bioelectronics, 2010, 25(10): 2212-2217.
［25］Chen P, Liu X, Wang B, et al. A biomimetic taste receptor cellbased biosensor for electrophysiology recording and acidic sensation ［J］. Sensors and Actuators B: Chemical, 2009, 139(2): 576-583.
［26］Misawa N, Mitsuno H, Kanzaki R, et al. Highly sensitive and selective odorant sensor using living cells expressing insect olfactory receptors［J］. Proceedings of the National Academy of Sciences, 2010, 107(35): 15340-15344.
［27］Lee SH, Ko HJ, Park TH. Realtime monitoring of odorantinduced cellular reactions using surface plasmon resonance［J］. Biosensors and Bioelectronics, 2009, 25(1): 55-60.
［28］Wu C, Chen P, Yu H, et al. A novel biomimetic olfactorybased biosensor for single olfactory sensory neuron monitoring ［J］. Biosensors and Bioelectronics, 2009, 24(5): 1498-1502.
［29］Wang TH, Hui GH, Deng SP. A novel sweet taste cellbased sensor［J］. Biosensors and Bioelectronics, 2010, 26(2): 929-934.
［30］Hui GH, Mi SS, Deng SP. Sweet and bitter tastants specific detection by the taste cellbased sensor［J］. Biosensors and Bioelectronics, 2012, 35(1): 429-438.
［31］Du L, Wu C, Liu Q, et al. Recent advances in olfactory receptorbasedbiosensors ［J］. Biosensors and Bioelectronics, 2013, 42: 570-580.
［32］Dacres H, Wang J, Leitch V, et al. Greatly enhanced detection of a volatile ligand at femtomolar levels using bioluminescence resonance energy transfer (BRET)［J］. Biosensors and Bioelectronics, 2011, 29(1): 119-124.
［33］Wetzel C H, Oles M, Wellerdieck C, et al. Specificity and Sensitivity of a Human Olfactory Receptor Functionally Expressed in Human Embryonic Kidney 293 Cells andXenopus Laevis Oocytes［J］. The Journal of neuroscience, 1999, 19(17): 7426-7433.
［34］Jacquier V, Pick H, Vogel H. Characterization of an extended receptive ligand repertoire of the human olfactory receptor OR17\|40 comprising structurally related compounds ［J］. Journal of Neurochemistry, 2006, 97(2): 537-544.
［35］Rhee HW, Lee SH, Shin IS, et al. Detection of kinase activity using versatile fluorescence quencher probes［J］. Angewandte Chemie, 2010, 122(29): 5039-5043.
［36］Hou Y, JaffrezicRenault N, Martelet C, et al. A novel detection strategy for odorant molecules based on controlled bioengineering of rat olfactory receptor I7 ［J］. Biosensor and Bioelectronics, 2007, 22(7): 1550-1555.
［37］Lee SH, Ko HJ, Park TH. Realtime monitoring of odorantinduced cellular reactions using surface plasmon resonance［J］. Biosensors and Bioelectronics, 2009, 25(): 55-60.［38］Kaiser L, GravelandBikker J, Steuerwald D, et al. Efficient cellfree production of olfactory receptors: detergent optimization, structure, and ligand binding analyses［J］. Proceedings of the National Academy of Sciences, 2008, 105(41): 15726-15731.
［39］Liu Q, Hu N, Ye W, et al. Extracellular recording of spatiotemporal patterning in response to odors in the olfactory epithelium by microelectrode arrays［J］. Biosensors and Bioelectronics, 2011, 27(1): 12-17.
［40］陈庆梅, 董琪, 胡靓, 等. 基于微电极阵列的离体嗅球振荡信号识别［J］. 科学通报, 2013, 58(19): 1851-1854.
［41］Chen QM, Dong Q, Hu L, et al. Discrimination of signal oscillations in the in vitro olfactory bulb with microelectrode array［J］. Chinese Science Bulletin, 2013, 5(24): 3015-3018.
［42］Liu Q, Zhang F, Zhang D, et al. Extracellular potentials recording in intact taste epithelium by microelectrode array for a taste sensor［J］. Biosensors and Bioelectronics, 2013, 43(2): 186-192.
［43］Zhang D, Zhang F, Zhang Q, et al. Umami evaluation in taste epithelium on microelectrode array by extracellular electrophysiological recording［J］. Biochemical and Biophysical Research Communications, 2013, 438(2): 334-339.
［44］Liu Q, Ye W, Yu H, et al. Olfactory mucosa tissuebased biosensor: A bioelectronic nose with receptor cells in intact olfactory epithelium ［J］. Sensors and Actuators B: Chemical, 2010, 146(2): 527-533.
［45］Liu Q, Ye W, Hu N, et al. Olfactory receptor cells respond to odors in a tissue and semiconductor hybrid neuron chip［J］. Biosensors and Bioelectronics, 2010, 26(4): 1672-1678.
［46］Zhang W, Li Y, Liu Q, et al. A novel experimental research based on taste cell chips for taste transduction mechanism ［J］. Sensors and Actuators B: Chemical, 2008, 131(1): 24-28.
［47］Wu C, Du L, Mao L, et al. A novel bitter detection biosensor based on light addressable potentiometric sensor［J］. Journal of Innovative Optical Health Sciences, 2012, 5(2):1-7. 
［48］Alfinito E, Pennetta C, Reggiani L. Olfactory receptorbased smell nanobiosensors: An overview of theoretical and experimental results ［J］. Sensors and Actuators B: Chemical, 2010, 146(2): 554-558.
［49］Liu Q, Wang H, Li H, et al. Impedance sensing and molecular modeling of an olfactory biosensor based on chemosensory proteins of honeybee［J］. Biosensors and Bioelectronics, 2013, 40(1): 174-179.
［50］Du L, Wu C, Peng H, et al. Piezoelectric olfactory receptor biosensor prepared by aptamerassisted immobilization ［J］. Sensors and Actuators B: Chemical, 2013, 187: 481-487.
［51］Wu C, Du L, Wang D, et al. A novel surface acoustic wavebased biosensor for highly sensitive functional assays of olfactory receptors ［J］. Biochemical and Biophysical Research Communications, 2011, 407(1): 18-22.
［52］Lehmkuhle MJ, Normann RA, Maynard EM. Trialbytrial discrimination of three enantiomer pairs by neural ensembles in mammalian olfactory bulb ［J］. Journal of Neurophysiology, 2006, 95(3): 1369-1379.
［53］You KJ, Ham HG, Lee HJ, et al. Odor discrimination using neural decoding of the main olfactory bulb in rats ［J］. IEEE Transactions on Biomedical Engineering, 2011, 58(5): 1208-1215.
［54］Brunjes PC, Illig KR, Meyer EA. A field guide to the anterior olfactory nucleus (cortex)［J］. Brain Research Reviews, 2005, 50(2): 305-335.
［55］Zhou J, Dong Q, Zhuang L, et al. Rapid odor perception in rat olfactory bulb by microelectrode array ［J］. Journal of Zhejiang University Science B, 2012, 13(12): 1015-1023.
［56］Dong Q, Du L, Zhuang L, et al. A novel bioelectronic nose based on brain-machine interface using implanted electrode recording in vivo in olfactory bulb［J］. Biosensors and Bioelectronics, 2013, 49: 263-269.
［57］Zhuang L, Hu N, Dong Q, et al. A high sensitive in vivo biosensing detection for odors by multiunit in rat olfactory bulb ［J］. Sensors and Actuators B: Chemical, 2013, 186: 308-314.
［58］Chen X, Gabitto M, Peng Y, et al. A gustotopic map of taste qualities in the mammalian brain ［J］. Science, 2011, 333(6047): 1262-1266.
［59］Sankaran S, Panigrahi S, Mallik S. Odorant binding protein based biomimetic sensors for detection of alcohols associated with salmonella contamination in packaged beef ［J］. Biosensors and Bioelectronics, 2011, 26(7): 3103-3109.
［60］Radhika V, ProikasCezanne T, Jayaraman M, et al. Chemical sensing of DNT by engineered olfactory yeast strain［J］. Nature Chemical Biology, 2007, 3(6): 325-330.
［61］Marshall B, Warr CG, De Bruyne M. Detection of volatile indicators of illicit substances by the olfactory receptors of Drosophila melanogaster ［J］. Chemical Senses, 2010, 35(7): 613-625.
［62］Liu Q, Zhang D, Zhang F, et al. Biosensor recording of extracellular potentials in the taste epithelium for bitter detection［J］. Sensors and Actuators B: Chemical, 2013, 176(1): 497-504. 
［63］Song HS, Kwon OS, Lee SH, et al. Human taste receptorfunctionalized field effect transistor as a humanlike nanobioelectronic tongue ［J］. Nano Letters, 2012, 13(1): 172-178.
［64］Feng Z, Chen W, Ye X, et al. A remote control training system for rat navigation in complicated environment ［J］. Journal of Zhejiang University Science A, 2007, 8(2): 323-330.