两种生物活性植骨材料对小鼠骨髓干细胞增殖和分化的影响

doi:10.3969/j.issn.0258-8021.2015.03.009

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摘要生长因子作用于拔牙后的牙槽窝，有可能阻止或减少牙槽骨的破坏吸收，从而维持牙槽骨原有的结构。为了奠定临床拔牙后、牙槽嵴位点保留的实验基础，本研究首先通过乳液交联法合成壳聚糖微球，负载生长因子BMP2及脂联素(APN)，再将其复合于小牛松质骨支架形成生物活性植骨材料。将两种生物活性材料与小鼠前成骨细胞(MC3T3E1)共培养，比较材料对细胞增殖和分化的影响。结果表明，负载BMP2的缓释微球表面更为光滑，微球平均直径为9634 μm。cck8结果表明，随着BMP2浓度的增加，细胞增殖速度较脂联素组增加更快。实时定量PCR结果表明，ALP、BMP2、OCN和Adipor2基因在APN处理组增加倍数高于BMP2组。WesternBlot结果表明，APN植骨材料更有利于小鼠前成骨细胞MC3T3E1的增殖和分化。

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	李德利¹李箐²
	3 王彤¹毋育伟¹卢松鹤¹

关键词 ：骨形态发生蛋白2(BMP 2), 脂联素(APN), 成骨细胞(MC3T3E1), 药物缓释体系

Abstract：More and more scientists focus on the growth factors that can improve the bone regeneration. The aim of the study was to prepare a bone scaffolds, and to investigate underlying molecular mechanisms in promoting osteogenesis on the socket of tooth extraction. Chitosan microspheres carrying two osteogenic factors, BMP2 or APN, were prepared by emulsion crosslinking, and then were mixed with ceramic bovine bone to form a novel drug delivery system. To compare the biological activity of the grafts, MC3T3E1 cells were seeded in the scaffold. The influence of the scaffold on cells proliferation and differentiation were investigated. Results show that the average diameter of the chitosan microspheres is 9634 μm. The scaffolds loading BMP2 or APN both contributed to the proliferation of osteoblasts MC3T3E1. Cells seeded in the scaffold loading BMP2 showed higher proliferation. However, cells seeded in the adiponectinloading scaffold showed a higher level in the expression of osteogenesisrelated proteins and genes compared with those in the BMP2 loading scaffold.

Key words： bone morphogenetic protein2(BMP2) adiponectin (APN) MC3T3E1 drug delivery

基金资助:首都市民健康项目培育项目(z2110005312001)

引用本文:

李德利¹李箐^2,3王彤¹毋育伟¹卢松鹤¹. 两种生物活性植骨材料对小鼠骨髓干细胞增殖和分化的影响[J]. 中国生物医学工程学报, 2015, 34(3): 323-329.

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http://cjbme.csbme.org/CN/10.3969/j.issn.0258-8021.2015.03.009 或 http://cjbme.csbme.org/CN/Y2015/V34/I3/323

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