基于壳聚糖的纳米材料在骨组织工程与再生医学中的研究进展

doi:10.3969/j.issn.0258-8021. 2013. 05.016

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摘要壳聚糖是目前发现的唯一与细胞外基质糖胺聚糖的化学结构相似的天然阳离子多聚糖，具有极为优良的生物相容性、生物可降解性和生物学活性。近年来，基于壳聚糖的纳米材料在组织工程中的研究较为广泛。对壳聚糖的纳米材料、壳聚糖复合纳米材料、壳聚糖纳米纤维和壳聚糖纳米粒子等在骨组织工程与再生医学中的研究进展进行回顾和阐述。近年来的研究显示，壳聚糖复合纳米材料生物支架、壳聚糖纳米纤维支架及包载具有骨诱导性的生物活性因子，以及外源基因的壳聚糖纳米粒子及纳米纤维，在骨组织工程与再生医学中具有良好的应用前景。

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	李晓静¹王新木² 董研^1*苟中入^3

关键词 ：壳聚糖, 纳米材料, 骨组织工程

Abstract：Chitosan is the only one natural cationic polysaccharide possessing similar chemical structures to extracellular glycosaminoglycan. Chitosan has excellent biocompatibility, biodegradability as well as biological activity. In the past decade, chitosanbased nanomaterials have been widely studied in bone tissue engineering. The present paper reviews the researches and application potentials of chitosan nanocomposites, chitosan nanofibers and chitosan nanoparticles in biomedical fields. Investigation results show that chitosan nanocomposites, chitosan nanofibrous scaffolds and chitosan nanoparticles loading biological active factors and exogenous genes have wide promising potentials for bone tissue engineering and regenerative medicine.

Key words： chitosan nanomaterials bone tissue engineering

基金资助:浙江省自然科学基金项目（Y2110260）；杭州市科技局科技发展计划项目（20090833B02）；卫生部省部共建项目（WKJ2009-2-033）

引用本文:

李晓静¹王新木² 董研^1*苟中入^3. 基于壳聚糖的纳米材料在骨组织工程与再生医学中的研究进展[J]. 中国生物医学工程学报, 2013, 32(5): 620-625.
LI Xiao Jing¹WANG Xin Mu²DONG Yan^1*GOU Zhong Ru³. Research Progress of Chitosanbased Nanomaterials in Bone Tissue Engineering and Regenerative Medicine. journal1, 2013, 32(5): 620-625.

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http://cjbme.csbme.org/CN/10.3969/j.issn.0258-8021. 2013. 05.016 或 http://cjbme.csbme.org/CN/Y2013/V32/I5/620

［1］Kessler MW, Grande DA. Tissue engineering and cartilage ［J］. Organogenesis, 2008, 4(1): 28-32.
［2］Venkatesan J, Kim SK. Chitosan composites for bone tissue engineering—an overview ［J］. Mar Drugs, 2010, 8(8): 2252-2266.
［3］Giannoudis PV, Dinopoulos H, Tsiridis E. Bone substitutes: an update ［J］. Injury, 2005, 36 (Suppl 3): S20-S27.
［4］Swetha M, Sahithi K, Moorthi A, et al. Biocomposites containing natural polymers and hydroxyapatite for bone tissue engineering ［J］. Int J Biol Macromol, 2010, 47(1): 1-4.［5］Francesko A, Tzanov T. Chitin, chitosan and derivatives for wound healing and tissue engineering ［J］. Adv Biochem Engin Biotechnol, 2010, 125: 1-27.
［6］Park BK, Kim MM. Applications of chitin and its derivatives in biological medicine ［J］. Int J Mol Sci, 2010, 11(12): 5152-5164.
［7］Yu Ting, Li Honghe, Zheng Xiaodong. Synergistic effect of chitosan and cryptococcus laurentii on inhibition of Penicillium expansum infections ［J］. Int J Food Microbiol, 2007, 114(3): 261-266.
［8］Valentine R, Athanasiadis T, Moratti S, et al. The efficacy of a novel chitosan gel on hemostasis and wound healing after endoscopic sinus surgery ［J］. Am J Rhinol Allergy, 2010, 24(1): 70-75.
［9］Kawai T, Yamada T, Yasukawa A, et al. Biological fixation of fibrous materials to bone using chitin/chitosan as a bone formation accelerator ［J］. J Biomed Mater Res B Appl Biomater, 2009, 88(1): 264-270.
［10］Teng SH, Lee EJ, Wang P, et al. Threelayered membranes of collagen/hydroxyapatite and chitosan for guided bone regeneration ［J］. J Biomed Mater Res B Appl Biomater, 2008, 87(1): 132-138.
［11］Lee EJ, Shin DS, Kim HE, et al. Membrane of hybrid chitosan–silica xerogel for guided bone regeneration ［J］. Biomaterials, 2009,30(5): 743-750.
［12］Kim K, Fisher JP. Nanoparticle technology in bone tissue engineering ［J］. J Drug TargeT, 2007, 15(4): 241-252.
［13］Kubinová , Syková E. Nanotechnologies in regenerative medicine ［J］. Minim Invasive Ther Allied Technol, 2010, 19(3): 144-156.
［14］孙康，王丽平. 壳聚糖静电纺纳米纤维的制备和特点［J］. 应用化学, 2011, 28(2): 123-130.
［15］Zeng Rong, Tu Mei, Liu hongwei, et al. Preparation, structure, drug release and bioinspired mineralization of chitosanbased nanocomplexes for bone tissue engineering ［J］. Carbohydr Polym, 2009, 78(1): 107-111.
［16］Zhao Jianhao, Han Wanqing, Chen Haodong, et al. Fabrication and in vivo osteogenesis of biomimetic poly(propylene carbonate) scaffold with nanofibrous chitosan network in macropores for bone tissue engineering ［J］. J Mater Sci Mater Med, 2011, 23(2): 517-525.
［17］Supaphol P, Suwantong O, Sangsanoh P, et al. Electrospinning of biocompatible polymers and their potentials in biomedical applications ［J］. Adv Polym Sci, 2011, 246: 213-239.
［18］Jang JH, Castano O, Kim HW. Electrospun materials as potential platforms for bone tissue engineering ［J］. Adv Drug Deliv Rev, 2009,61(12): 1065-1083.
［19］Ki CS, Park SY, Kim HJ, et al. Development of 3D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration ［J］. Biotechnol Lett, 2007, 30(3): 405-410.
［20］Vaquette C, CooperWhite JJ. Increasing electrospun scaffold pore size with tailored collectors for improved cell penetration ［J］. Acta Biomater, 2011, 7(6): 2544-2557.
［21］Lee JB, Jeong SI, Bae MS, et al. Highly porous electrospun nanofibers enhanced by ultrasonication for improved cellular infiltration ［J］. Tissue Eng Part A, 2011, 17(21-22): 2695-2702.
［22］Liverani L, Roether JA, Nooeaid P, et al. Simple fabrication technique for multilayered stratified composite scaffolds suitable for interface tissue engineering ［J］. Mater Sci Eng A Struct Mater, 2012, 557: 54-58.
［23］Wang Jianwen., Chen Chingyi, Kuo Yiming. Effect of experimental parameters on the formation of chitosanpoly(acrylic acid) nanofibrous scaffolds and evaluation of their potential application as DNA carrier ［J］. J Appl Polym Sci, 2010, 115(3): 1769-1780.
［24］Grenha, A. Chitosan nanoparticles: a survey of preparation methods ［J］. J Drug Target, 2012, 20(4): 291-300.
［25］Kalpana N, Shailendra KS, Dina NM. Chitosan nanoparticles：a promising system in novel drug delievry ［J］. Chem Pharm Bull, 2010, 58(11): 1423-1430.
［26］Mishra D, Bhunia B, Banerjee I, et al. Enzymatically crosslinked carboxymethylchitosan/gelatin/nanohydroxyapatite injectable gels for in situ bone tissue engineering application ［J］. Mater Sci Eng C Mar Biol Appl, 2011, 31(7): 1295-1304.
［27］Couto D, Hong Zhongkui, Mano JF. Development of bioactive and biodegradable chitosanbased injectable systems containing bioactive glass nanoparticles ［J］. Acta Biomater, 2009, 5(1): 115-123.
［28］刘顺振，侯玉东. 骨组织工程支架材料的研究进展及临床应用［J］. 中国组织工程研究与临床康复, 2011, 15(42): 7911-7914.
［29］Shin SY, Park HN, Kim KH, et al. Biological evaluation of chitosan nanofiber membrane for guided bone regeneration ［J］. J Periodontol, 2005, 76(10): 1778-1784.
［30］Cho WJ, Kim JH, Oh SH, et al. Hydrophilized polycaprolactone nanofiber meshembedded poly(glycoliccolactic acid) membrane for effective guided bone regeneration ［J］. J Biomed Mater Res A, 2009, 91(2): 400-407.
［31］Chen Zonggang, Wei Bo, Mo Xiumei, et al. Mechanical properties of electrospun collagen–chitosan complex single fibers and membrane ［J］. Mater Sci Eng C Mar Biol Appl, 2009, 29(8): 2428-2435.
［32］Norowski PA, Mishra S, Adatrow PC, et al. Suture pullout strength and in vitro fibroblast and RAW 2647 monocyte biocompatibility of genipin crosslinked nanofibrous chitosan mats for guided tissue regeneration ［J］. J Biomed Mater Res A, 2012, 100(11): 2890-2896.
［33］Datta P, Dhara S, Chatterjee J. Hydrogels and electrospun nanofibrous scaffolds of Nmethylene phosphonic chitosan as bioinspired osteoconductive materials for bone grafting ［J］. Carbohydr Polym, 2012, 87(2): 1354-1362.
［34］TheinHan WW, Misra RDK. Biomimetic chitosannanohydroxyapatite composite scaffolds for bone tissue engineering ［J］. Acta Biomater, 2009, 5(4): 1182-1197.
［35］Wang Shaofeng, Shen Lu, Zhang Weide, et al. Preparation and mechanical properties of chitosan/carbon nanotubes composites ［J］. Biomacromolecules, 2005, 6(6): 3067-3072.
［36］Jafarkhani M, Fazlali A, Moztarzadeh F, et al. Mechanical and structural properties of polylactide/chitosan scaffolds reinforced with nanocalcium phosphate ［J］. Iran Polym J, 2012, 21(10): 713-720.
［37］Venkatesan J, Ryu B, Sudha PN, et al. Preparation and characterization of chitosan–carbon nanotube scaffolds for bone tissue engineering ［J］. I J Biol Macromol, 2012, 50(2): 393-402.
［38］Ma Xing, Wang Yang, Guo Hun, et al. Nanohydroxyapatite/chitosan spongelike biocomposite for repairing of rat calvarial criticalsized bone defect ［J］. J Bioact Compat Polym, 2011, 26(4): 335-346.
［39］Pattnaik S, Nethala S, Tripathi A, et al. Chitosan scaffolds containing silicon dioxide and zirconia nano particles for bone tissue engineering ［J］. Int J Biol Macromol, 2011, 49(5): 1167-1172.
［40］王玮，尹庆水，张余. 重组人骨形态发生蛋白2壳聚糖纳米微球的制备及体外细胞毒性［J］. 中国组织工程研究与临床康复, 2011, 15(25): 4611-4614.
［41］黄鑫, 孟建国, 刘建，等. rhBMP2壳聚糖微球的制备及体外检测［J］. 中国矫形外科杂志, 2009 ，17(15): 1172-1174.
［42］Hou Juan, Wang Jing, Cao Lingyan, et al. Segmental bone regeneration using rhBMP2loaded collagen/chitosan microspheres composite scaffold in a rabbit model ［J］. Biomed Mater, 2012, 7(3): 035002.
［43］Rieux A, Ucakar B, Mupendwa BPK, et al. 3D systems delivering VEGF to promote angiogenesis for tissue engineering ［J］. J Control Release, 2011, 150(3): 272-278.［44］Jayakumar R, Chennazhi KP, Muzzarelli RAA, et al. Chitosan conjugated DNA nanoparticles in gene therapy ［J］. Carbohydr Polym, 2010, 79(1):1-8.
［45］Nie H, Ho ML, Wang CK, et al. BMP2 plasmid loaded PLGA/HAp composite scaffolds for treatment of bone defects in nude mice ［J］. Biomaterials,2009, 30(5): 892-901.［46］卢华定，吕璐璐，赵慧清. 转化生长因子B1基因缓释的壳聚糖纳米粒制备及体外检测［J］. 中国组织工程研究, 2012, 16(12): 2110-2124.
［47］Lai WF, Lin MCM. Nucleic acid delivery with chitosan and its derivatives ［J］. J Control Release, 2009, 134(3): 158-168.
［48］Yu Wenyuan. A preliminary study on the evaluation of a novel gene delivery vectorTACS in vitro and in vivo via coexpressing hVEGF and hBMPgenes to repair criticalsize rabbit bone defects ［J］. J Pharm Pharmacol, 2012, 6(5): 343-351.

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