TEMPO-NaBr-NaClO 体系对细菌纤维素的氧化过程研究

doi:10.3969/j.issn.0258-8021. 2013. 06.09

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摘要细菌纤维素是具有天然纳米网状结构的支架材料，选择性催化氧化可改善其降解性能。以NaClO、TEMPO和反应时间为变量，研究2,2,6,6-四甲基哌啶-1-氧化物自由基(TEMPO)/NaBr/NaClO体系对细菌纤维素的氧化。结果表明，在试验条件范围内，体系初始阶段的反应速率随NaClO用量的增加而减小；体系pH值是影响此阶段反应速率的主要因素且体系最大反应速率出现在pH＝10.50～11.00之间；TEMPO用量对此阶段反应速率的影响不明显。对整个反应过程而言，体系的反应速率和不溶性产物的羧基含量都随NaClO用量的增加而增大，其中NaClO用量在1～8 mL之间时，都呈现良好的线性关系；二者都随催化剂TEMPO用量的增加而增大。不溶性产物的羧基含量随反应时间的延长先逐渐减小后保持相对稳定在0.70～0.75 mol/kg。在不同反应阶段，TEMPO/NaBr/NaClO体系对BC的氧化规律存在明显差异，BC独特的结构特点可能是导致这种差异的主要原因。

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	廖世波¹奚廷斐¹
	2
	4赖琛^2廖世玉³黄涛⁴王甩艳^4

关键词 ：细菌纤维素, 2,2,6,6-四甲基哌啶-1-氧化物自由基, 选择性氧化, 羧基含量

Abstract：Bacterial cellulose (BC) is a kind of biomedical scaffold with nano network, and its biodegradation can be improved through selective catalytic oxidation. The TEMPO-mediated oxidation of BC was investigated when the amount of NaClO, TEMPO and the reaction time changed. The experimental results reveal that, in the range of test conditions, the initial reaction rate is inversely proportional to the amount of NaClO; the pH of system is one of the main factors which influence this stage reaction rate, and the maximum reaction rate appears in pH＝10.50～11.00; while the amount of TEMPO has no obvious effect on it. For the whole reaction process, both the reaction rate and waterinsoluble fractions' carboxylic content are proportional to the amount of NaClO, and they have a good linear relationship when the dosage of 0.6 mol/L NaClO is during 1～8 mL; both of them are also proportional to the amount of TEMPO. The waterinsoluble fractions' carboxylic content gradually decreases at first and then remains stable at 0.70～0.75 mol/kg with increasing oxidation time. In conclusion, the rule of the TEMPO-mediated oxidation of BC has significant differences in different stages, and the main cause could be its unique nanostructure and properties.

Key words： bacterial cellulose TEMPO selective oxidation carboxylic content

基金资助:国家重大科学研究计划项目（2012CB933603）；浙江省新苗人才计划项目（2012R413046）；深圳市科技计划项目（JC200903170498A）

引用本文:

廖世波¹奚廷斐^1,2,4*赖琛^2*廖世玉³黄涛⁴王甩艳^4
. TEMPO-NaBr-NaClO 体系对细菌纤维素的氧化过程研究[J]. 中国生物医学工程学报, 2013, 32(6): 699-707.
LIAO Shi Bo¹XI Ting Fei^1,2,4*LAI Chen^2*LIAO Shi Yu³HUANG Tao⁴WANG Shuai Yan^4. Study on TEMPO-mediated Oxidation of Bacterial Cellulose. journal1, 2013, 32(6): 699-707.

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http://cjbme.csbme.org/CN/10.3969/j.issn.0258-8021. 2013. 06.09 或 http://cjbme.csbme.org/CN/Y2013/V32/I6/699

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