Study on Preparation and Biocompatibility of Novel Porous PCL/CPC Scaffolds
1 School of Stomatology, Tongji University, Shanghai 200072, China
2 Department of Stomatology, Hangzhou First People′s Hospital, Hangzhou 310006, China
3 Institute of Advanced Materials & Nano Biomedicine, Tongji University, Shanghai 200072, China
4 Department of Stomatology, Shanghai East Hospital Affiliated with Tongji Univrsity, Shanghai 200120, China
Abstract：To investigate the preparation and biocompatibility of porous polycaprolactone/calcium phosphate cement (PCL/CPC) scaffolds in uses for bone marrow stromal cells (BMSCs), the scaffolds were fabricated by solution blending method, and water soluble salt crystals were used as pore-forming agent. The dynamic mechanical test (DMA) was conducted using Q800 dynamic mechanical analyzer, with the porosity measured by drainage method. BMSCs were implanted and cultured with PCL/CPC scaffolds in vitro, PCL and CPC scaffolds were taken as controls. The attachment, proliferation and differentiation of BMSCs were detected through morphological characters, growth curve, the activity of alkaline phosphatase (ALP) and osteocalcin (OC) and entopic bone formation in nude mice. Results showed that, storage modulus of the composite scaffold reached the maximum when the ratio of PCL to CPC was 7:3, the pore size of the material was 250~350 μm, and the porosity of the scaffolds reached 70%~80%. BMSCs attached to and grew better on the surface of porous PCL/CPC and CPC scaffolds than PCL scaffolds (P＜0.05), and BMSCs proliferate, and function more actively on porous PCL/CPC and CPC scaffolds than on PCL scaffolds (P＜0.05). More bone form in nude mice of PCL/CPC 13.78%±1.60% and CPC scaffolds 15.29%±1.20% than PCL scaffolds 7.56%±2.20% (P＜0.05). In summary, PCL/CPC scaffolds have good biocompatibility for BMSCs to fabricate tissue-engineered bone in vitro.
