聚左乳酸己内酯-胶原蛋白静电纺人工血管的降解研究

doi:10.3969/j.issn.0258-8021. 2014. 06.015

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摘要探讨聚左旋乳酸己内酯-胶原蛋白构建的可降解人工血管材料的降解规律以及生物相容性。聚左旋乳酸己内酯-胶原蛋白混合溶液通过静电纺丝技术制成静电纺人工血管材料作为实验组(n=40)，市售聚四氟乙烯人工血管材料作为对照组(n=40)，分别植入兔背部脊柱两侧肌肉，于术后10、30、90、180 d取材，行形态学、组织学观察；并在400 倍光学显微镜视野下进行中性粒细胞、巨噬细胞和淋巴细胞的分类计数。结果镜下聚左旋乳酸己内酯胶原蛋白组材料周围组织炎症反应轻，术后90 d材料碎裂，纤维组织长入材料网孔间隙内，逐步取代材料部位，形成囊状纤维膜；术后180 d材料基本降解殆尽，植入区域组织重塑后形态接近正常组织。聚四氟乙烯组材料周围组织炎症反应轻，术后180 d仍基本保持原有结构，材料周围有薄层纤维组织膜包绕。细胞计数结果提示植入后不同时期两组材料引起的细胞反应类型和反应趋势相同；仅术后10 d时实验组中性粒细胞数量较对照组增多(2244±372 vs 1922±181 个/025 mm2, P<005)，其余两组各期各类细胞均无明显差异(P>005)。实验材料降解时间为3～6月，具有生物相容性好、降解规律适宜的良好生物学性能，有作为血管替代物的潜在可能性。

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	汪涛¹ 陈剑锋² 赵珺^1*吴桐²莫秀梅²梅家

关键词 ：聚左旋乳酸己内酯-胶原蛋白, 降解, 生物相容性

Abstract：To evaluate the biodegradation and biocompatibility of poly (Llactic acidcoεcaprolactone) (P(LLACL))/collagen graft, P (LLACL) collagen graft scaffolds were made through electrospinning. The prepared scaffolds were set as experimental group (n=40) and the commercial artificial vessel made
of ePTFE was set as control group (n=40). The scaffold samples were implanted in the left dorsal muscle of rabbits, while ePTFE graft materials were implanted in the opposite side. On day 10, 30, 90 and 180 after the implantation, the samples were harvested. The histological staining was applied to observe the degradation of the material and tissue reaction. The number of neutrophils, macrophages and lymphocytes were counted at the high magnification (400×) using the optical microscope. Under the microscope, slight tissue reaction was revealed in the area of P (LLACL)/collagen material. On day 90, the material broken was observed. Some connective fibrous tissue was revealed in the material, a capsulelike membrane was established over the area. During the process of degradation, most of the materials were absorbed within 180 days. The structure and distribution of the tissue cells was highly similar to that of the surrounding area, indicating that the remodeling of the embedded tissue was almost finished. The ePTFE materials were revealed still maintaining the structure encompassed in a thin layer of fibrous connective tissue. The result of cell count showed that the type and trend of cell reaction had no difference after the different stages of postoperation. However, the count of neutrophile granulocyte in experimental group was more than that in the control group on day 10 (2244±372 vs 1922±181 /025 mm2, P<005), while no difference of various types of cells was observed between the two groups post operation. The degeneration of P (LLACL)/collagen material was 90-180 days, which is a satisfying biological performance in the degradation and biocompatibility.

Key words： P (LLACL)/collagen biocompatibility biodegradation

引用本文:

汪涛¹ 陈剑锋² 赵珺^1*吴桐²莫秀梅²梅家. 聚左乳酸己内酯-胶原蛋白静电纺人工血管的降解研究[J]. 中国生物医学工程学报, 2014, 33(6): 746-752.
WANG Tao¹CHEN Jian Feng²ZHAO Jun^1*WU Tong² MO Xiu Mei²MEI Jia Cai^1. Biodegradation of Electrospun Poly (Llactic acidcoεcaprolactone)/Collagen Composite Vascular Grafts. journal1, 2014, 33(6): 746-752.

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