Preparation and in vitro Characteristics of Collagen/ Fibrin/BSA Microspheres Complex Tissue Engineering Scaffolds
1 School of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, China
2 School of Pharmacy, Xinxiang Medical University, Xinxiang 453003, China
3 Institute of Biomedical Engineering , Chinese Academy of Medical Sciences & Peking Union Medical College , Tianjin 300192, China
Abstract：In order to overcome the problem of nutrition insufficiency and inhomogeneous distribution in tissue engineering scaffolds, a scaffold (SCFM) composed of collagen/fibrin/BSA microspheres was prepared and the features were characterized, especially the release behaviors of BSA. Porous collagen scaffolds for tissue engineering were fabricated by freezedrying and crosslinking. BSA loaded microspheres mixed with fibrin into collagen scaffolds for SCFM. The physiochemical properties of the scaffolds, such as surface morphology, porosity, pore sizes，mechanical function, and BSA contents in the scaffold were measured. SEM results showed that collagen scaffolds exhibited a highly porous and interconnected structure, the pore size of the material was (130±45) μm. Results of in vitro release tests revealed the BSA released from SCFM was 72.36%±3.48% of the original amount of BSA encapsulated after 144 h. The cumulative release of BSA from collagen/fibrin/BSA scaffolds (SCFB) within 48 h was 75.20%±2.74 %. There were no significant changes in the further prolonged period after 48 h. The SCFM achieved a relatively constant release and prolonged BSA release properties as compared to the SCFB. It was found that BSA concentration inside of SCFMwas higher than the external release medium before 96 h. The conformations of the BSA from the SCFM did not have significant changes. The SCFMobtained sustaining release ability and can maintain a homogeneous concentration of the BSA inside the scaffold for a long time. Therefore it provides a kind of promising scaffolds for tissue engineering.