鱼皮胶原蛋白海绵组织相容性的体内实验研究

doi:10.3969/j.issn.0258-8021.2014. 01.011

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摘要为评价鱼皮胶原蛋白海绵作为植入型生物材料的组织相容性，对草鱼皮胶原海绵的体内细胞附着性、免疫原性、致炎性、稳定性与形变进行了实验研究，并与猪皮胶原海绵相比较。鱼皮酸溶性胶原蛋白（FA）、鱼皮酶溶性胶原蛋白（FP）和猪皮酶溶性胶原蛋白（PP）海绵均为细纤维组成疏松多孔显微结构。2 mm× 2 mm × 8 mm杆状胶原海绵植入SD大鼠后肢（每组n=6）长收肌1周，3种材料内部均有大量有核细胞入驻。比较炎性细胞浸润面积，FP组（（7.93 ± 0.77）mm2）与PP组（（7.49 ± 1.03）mm²）没有显著差异，FA组（（9.81 ± 1.42）mm²）与其他两组具有显著性差异。FP组材料截面变形但边缘清晰，疏松纤维结构完整。FA组材料边缘模糊，内部见散在断裂纤维。PP组材料边缘已失去，未见纤维结构。昆明小鼠（每组 n=5）接受胶原蛋白溶液注射后检测血清特异性IgG水平，酶联免疫吸光度值FA组（0.602 ± 0.036）、FP组（0.518 ± 0.019）、PP组（0.390 ± 0.085），仅FA组与PP组间有显著性差异。以上结果显示三种材料均适于细胞附着，FP的免疫原性及致炎性接近于PP，FP海绵体内稳定性好于FA海绵与PP海绵。实验结果表明3种检测材料中鱼皮酶溶性胶原海绵（FP）具有组织相容性优势。

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	方成^1*汪海波²梅智强

关键词 ：草鱼, 猪, 皮肤, 胶原, 组织相容性

Abstract：This study investigated the cellular permeability, immunogenicity, inflammatory inductivity, biodegradation and deformation of grass carp skin collagen sponge  in vivo to evaluate the histocompatibility and compared with pigskin collagen sponge. Fishskin acidsoluble collagen (FA), fishskin pepsinsoluble collagen (FP) and pigskin pepsinsoluble collagen (PP) sponges were prepared by selfassembly and had highly open porous structure with microfibrous. The collage sponges with 2×2×8 mm rod shape were implanted in the adductor longus muscles (n=6 each group) of SD rats. After 1 week, three kinds of materials were penetrated by nucleated cells. There was not significant difference of the inflammatory areas between group FP (7.93±0.77 mm²) and PP (7.49±1.03 mm²), but the area of group FA (9.81±1.42 mm²) was significantly greater. The cross sections of FP pallets were deformed but the borders were clear and the fibrous networks were complete. The fibrous networks of FA pallets were disrupted and the PP pallets’ were disappeared. Assessing the amount of specific IgG antibodies in sera of KM mice (n=5 each group) immunized with collagen solution by ELISA, the OD values of group FA, FP, and PP were (0.602±0.036), (0.518±0.019) and (0.390 ± 0.085). There was only significant difference of the amount of specific IgG between group FA and PP. These results indicated that the three kinds of materials are suitable for cellular immigration. The immune and inflammatory responses to FP sponge are similar to PP sponge. The biological stability of FP sponge is superior to that of FA and PP sponges. Experimental results suggest that fishskin pepsinsoluble collagen sponge has histocompatible advantages among the three kinds of materials.

Key words： Ctenopharyngodon idellus pig skin collagen histocompatibility

基金资助: 国家自然科学基金（21076166）；湖北省高等学校优秀中青年科技创新团队计划项目( T201208)；武汉市科技局应用基础研究项目（2013020501010177）

引用本文:

方成^1*汪海波²梅智强FANG Cheng ^1*WANG HaiBo²MEI ZhiQiang¹XIAO XianJie¹LIU Li¹WANG Jia. Evaluating the Histocompatibility of Fishskin Collagen Sponges as Biomaterial in vivo. journal1, 2014, 33(2): 212-217.

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http://cjbme.csbme.org/CN/10.3969/j.issn.0258-8021.2014. 01.011 或 http://cjbme.csbme.org/CN/Y2014/V33/I2/212

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