Abstract：This study investigated the cellular permeability, immunogenicity, inflammatory inductivity, biodegradation and deformation of grass carp skin collagen sponge in vivo to evaluate the histocompatibility and compared with pigskin collagen sponge. Fishskin acidsoluble collagen (FA), fishskin pepsinsoluble collagen (FP) and pigskin pepsinsoluble collagen (PP) sponges were prepared by selfassembly and had highly open porous structure with microfibrous. The collage sponges with 2×2×8 mm rod shape were implanted in the adductor longus muscles (n=6 each group) of SD rats. After 1 week, three kinds of materials were penetrated by nucleated cells. There was not significant difference of the inflammatory areas between group FP (7.93±0.77 mm2) and PP (7.49±1.03 mm2), but the area of group FA (9.81±1.42 mm2) was significantly greater. The cross sections of FP pallets were deformed but the borders were clear and the fibrous networks were complete. The fibrous networks of FA pallets were disrupted and the PP pallets’ were disappeared. Assessing the amount of specific IgG antibodies in sera of KM mice (n=5 each group) immunized with collagen solution by ELISA, the OD values of group FA, FP, and PP were (0.602±0.036), (0.518±0.019) and (0.390 ± 0.085). There was only significant difference of the amount of specific IgG between group FA and PP. These results indicated that the three kinds of materials are suitable for cellular immigration. The immune and inflammatory responses to FP sponge are similar to PP sponge. The biological stability of FP sponge is superior to that of FA and PP sponges. Experimental results suggest that fishskin pepsinsoluble collagen sponge has histocompatible advantages among the three kinds of materials.
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