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中国生物医学工程学报
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胶原/纤维蛋白胶/载BSA微球复合支架的制备及体外性能研究
1新乡医学院生命科学技术学院,新乡 453003
2 新乡医学院药学院,新乡 453003
3 中国医学科学院北京协和医学院生物医学工程研究所,天津 300192
Preparation and in vitro Characteristics of Collagen/ Fibrin/BSA Microspheres Complex Tissue Engineering Scaffolds
1 School of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, China
2 School of Pharmacy, Xinxiang Medical University, Xinxiang 453003, China
3 Institute of Biomedical Engineering , Chinese Academy of Medical Sciences & Peking Union Medical College , Tianjin 300192, China
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摘要 为改善组织工程支架材料内部营养供应不足及分布不均的问题,制备胶原/纤维蛋白胶/载BSA微球复合支架(SCFM)并研究其理化性质及其BSA释放行为。本研究中采用戊二醛交联并冷冻干燥制备多孔胶原海绵支架,纤维蛋白与载BSA的PLGA微球混合后加入凝血酶,注入胶原膜中制备复合支架,研究支架形态学、孔隙率、机械强度等理化性质;通过BCA试剂盒法测定支架内部不同部位BSA含量及释放行为,圆二色谱法检测BSA的二级结构。扫描电镜结果显示制备的胶原支架孔径分布均匀,孔径平均(130±45) μm;相对于胶原/纤维蛋白胶/BSA支架(SCFB),在体外释放48 h时累积释放率为75.20%±2.74 %,随后BSA即不再显著增加;而支架SCFM中的BSA释放时间延长,持续释放144 h,测定其累积释放量为72.36%±3.48 %;测定支架SCFM代表性的3个部位的BSA含量均匀,在释放96 h内均高于支架外BSA含量,且圆二色谱结果表明BSA二级结构未见异常。支架SCFM可长时间维持支架内部BSA含量,且持续均匀释放,是理想的组织工程支架材料。
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陈红丽1 吕洁丽2 南文滨1&nb
关键词 胶原纤维蛋白PLGA微球组织工程支架    
Abstract:In order to overcome the problem of nutrition insufficiency and inhomogeneous distribution in tissue engineering scaffolds, a scaffold (SCFM) composed of collagen/fibrin/BSA microspheres was prepared and the features were characterized, especially the release behaviors of BSA. Porous collagen scaffolds for tissue engineering were fabricated by freezedrying and crosslinking. BSA loaded microspheres mixed with fibrin into collagen scaffolds for SCFM. The physiochemical properties of the scaffolds, such as surface morphology, porosity, pore sizes,mechanical function, and BSA contents in the scaffold were measured. SEM results showed that collagen scaffolds exhibited a highly porous and interconnected structure, the pore size of the material was (130±45) μm. Results of in vitro release tests revealed the BSA released from SCFM was 72.36%±3.48% of the original amount of BSA encapsulated after 144 h. The cumulative release of BSA from collagen/fibrin/BSA scaffolds (SCFB) within 48 h was 75.20%±2.74 %. There were no significant changes in the further prolonged period after 48 h. The SCFM achieved a relatively constant release and prolonged BSA release properties as compared to the SCFB. It was found that BSA concentration inside of SCFMwas higher than the external release medium before 96 h. The conformations of the BSA from the SCFM did not have significant changes. The SCFMobtained sustaining release ability and can maintain a homogeneous concentration of the BSA inside the scaffold for a long time. Therefore it provides a kind of promising scaffolds for tissue engineering.
Key wordscollagen    fibrin    PLGA microspheres    tissue engineering scaffolds
    
基金资助:河南省教育厅自然科学研究计划项目(2011B310006);河南省科技攻关项目(122102210148,122102310195)
引用本文:   
陈红丽1     吕洁丽2   南文滨1&nb. 胶原/纤维蛋白胶/载BSA微球复合支架的制备及体外性能研究[J]. 中国生物医学工程学报, 2014, 33(1): 79-85.
CHEN Hong Li1   LV Jie Li2    NAN Wen Bin1    LIU Rui1     ZHANG Hui2GUO Wei Yu. Preparation and in vitro Characteristics of Collagen/ Fibrin/BSA Microspheres Complex Tissue Engineering Scaffolds. journal1, 2014, 33(1): 79-85.
链接本文:  
http://cjbme.csbme.org/CN/10.3969/j.issn.0258-8021. 2014.01.012     或     http://cjbme.csbme.org/CN/Y2014/V33/I1/79
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