新型醛基化海藻酸钠-肝素复合涂层的制备及性能评价

doi:10.3969/j.issn.0258-8021.2013.01.011

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摘要研制一种新型基于多醛基海藻酸钠和肝素的复合涂层，并就其生物相容性和血液相容性进行实验和评价。通过高碘酸钠氧化海藻酸及重氮化处理肝素获得多糖分子片段，多糖分子片段通过共价交联固定于体外循环管路表面。通过红外及紫外扫描对固定的多糖分子片段进行定性及定量分析；通过血小板粘附实验及蛋白粘附实验对涂层管道的血液相容性进行评价；通过表面接触角实验评价涂层管路的亲水活性；通过凝血功能检测评价管路表面的抗凝血活性。结果显示：氧化海藻酸及重氮化处理后肝素在在管道表面固定确实；涂层组与未涂层组相比，血小板粘附量及血栓形成量均显著减少（P＜0.05）；肝素涂层组（LMNH）组与空白对照（C）组相比，蛋白粘附量无显著差异（P＞0.05）；海藻酸钠涂层（OSA）组、双涂层（OSA.LMNH）组与LMNH组相比，蛋白粘附量均显著减少（P<0.05）；LMNH组、OSA.LMNH组APTT及TT时间均显著延长（P<0.05），抗凝血性能优良；OSA组与C组相比，APTT时间显著延长（P＜0.05）具有一定的抗凝血性能；OSA组、OSA.LMNH组与C组相比，表面接触角明显减小（P＜0.05）；LMNH组与C组相比，表面接触角无显著差异（P＞0.05）。实验结果表明，OSA涂层物具有更优的亲水性和抗蛋白粘附性，LMNH涂层物具有更优的抗凝血性能，多糖片段复合涂层综合了单涂层的优点，生物相容性和血液相容性较单涂层均明显改善。

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	高文卿¹
	2李彤^1*于美丽³段大为¹胡晓旻¹周淑芬¹李鑫¹ 刘东¹

关键词 ：海藻酸钠, 肝素, 终点固定, 生物相容性, 血液相容性

Abstract：The aim of this work is to prepare a new composite coating based on sodium alginate and heparin with aldehyde groups, and to evaluate the biocompatibility and hemocompatibility of extracorporeal circulation loop. The loop samples were divided into four groups: (1) blank control (C), (2) sodium alginate (OSA), (3) heparin (LMNH), and (4) sodium alginate plus heparin (OSA.LMNH). The OSA and LMNH were bound to the surface of extracorporeal circulation loop by covalent linkage. Qualitative and quantitative analysis of polysaccharide fragments were conducted with FTIR and UV spectroscopy, the hemocompatibility of coated loop was evaluated by platelet adhesion, coagulation examination and protein adhesion, the hydrophily of coated loop was evaluated by surface contact angle. Experimental results showed that the surface of extracorporeal circulation loop was coated with OSA and LMNH. The platelet adhesion and thrombosis for the coated group were both decreased significantly compared with the noncoated group (P<0.05). The protein adhesion of LMNH group has no significantly difference with the noncoated group. The protein adhesion of OSA group and OSA.LMNH group significantly decreased compared with LMNH group (P＜0.05). The anticoagulant property of LMNH group and OSA.LMNH group is good with significantly prolonged time of APTT and TT. The APTT time of OSA group is significantly prolonged compared with C group (p＜0.05). The surface contact angles of OSA and OSA.LMNH group is significantly decreased compared with C group(p＜0.05). The surface contact angle of LMNH group has no significantly difference with C group. Composite coating of polysaccharide fragments has improved anticoagulant property in reference to the control, and the hemocompatibility of the composite coating was obviously improved compared with single coating.

Key words： sodium alginate heparin endpoint attachment biocompatibility hemocompatibility

基金资助:天津市科技支撑计划重点项目（11ZCGYSY02000），天津市科委项目（09JCYBJC27600）

引用本文:

高文卿^1,2李彤^1*于美丽³段大为¹胡晓旻¹周淑芬¹李鑫¹ 刘东¹
. 新型醛基化海藻酸钠-肝素复合涂层的制备及性能评价[J]. 中国生物医学工程学报, 2013, 32(1): 78-85.
GAO Wen Qing^1,2LI Tong ^1*YU Mei Li³DUAN DaWei ^1HU Xiao Min^1ZHOU Shu Fen¹LI Xin¹LIU Dong

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http://cjbme.csbme.org/CN/10.3969/j.issn.0258-8021.2013.01.011 或 http://cjbme.csbme.org/CN/Y2013/V32/I1/78

［1］RApostolakis EE, Koletsis EN, Baikoussis NG, et al. Strategies to prevent intraoperative lung injury during cardiopulmonary bypass［J］. J Cardiothorac Sury,2010,11(5):1.
［2］Suzuki Y, Daitoku K, Minakawa M, et al. Poly2methoxyethylacrylatecoated bypass circuits reduce activation of coagulation system and inflammatory response in congenital cardiac surgery［J］. J Artif Organs,2008,11(3):111-116.
［3］FinneWistrand A, Albertsson AC, Kwon OH, et al. Resorbable scaffolds from three different techniques: electrospun fabrics, saltleaching porous films, and smooth flat surfaces［J］. Macromol Biosci,2008,8(10):951-959.
［4］Sperling C,Houska M, Brynda E, et al. In vitro
hemocompatibility of albuminheparin multilayer coatings on polyethersulfone prepared by the layerbylayer technique［J］. J Biomed Mater Res, 2006,76(4):681-689.
［5］WeberN, Wendel P, Ziemer G. Hemocompatibility of heparincoated surfaces and the role of selective plasma protein adsorption［J］. Biomaterials, 2002, 23(2): 429-439.
［6］柏淑颖,朱德明,王伟. 涂层技术在体外循环装置中的应用及意义［J］. 中国体外循环杂志,2009,7(2):124-128.
［7］Larm O, Larsson R, Olsson P. A new nonthrombogenic surface prepared by selective covalent binding of heparin via a modified reducing terminal residue［J］. Biomater Med Devices Artif Organs, 1983,11(2-3):161-234.
［8］Andersson LO, Hoffman J,et al. Mechanisms of anticoagulant effects of some sulphated polysaccharides［J］. Thromb Res,1982,28(6):741-748.
［9］Smith PK, Mallia AK, Hermanson GT. Colorimetric method for the assay of heparin content in immobilized heparin preparations ［J］. Analytical Biochemistry, 1980, 109(2):466-473.
［10］Dubois M，Gilles KA，Hamilton JK，et al. Colorimetric method for determination of sugars and related substances［J］.Analytical Chemistry, 1956, 28(3) : 350-356.
［11］Ishihara K, Fukumoto K, Iwasaki Y, et al. Modification of polysulfone with phospholipid polymer for improvement of the blood compatibility. Part 1. Surface characterization［J］.Biomaterials 1999, 20(17):1545–1551.
［12］Gunaydin S. Clinical significance of coated extracorporeal circuits: a review of novel technologies ［J］.Perfusion, 2004,19(1):33-41.
［13］Petitou M, Barzu T, Herault JP et al. A unique trisaccharide sequence in heparin mediates the early step of antithrombin IIactivation［J］.Glycobiology,1997,7(3):323-327.
［14］Santerre JP，Woodhouse K，Laroche G，et al. Understanding the biodegradation of polyurethanes: from classical implants to tissue engineering materials［J］. Biomaterials, 2005,26(35): 7457-7470.
［15］Jeffrey FW, Wielders SJ, Willems GM, et al. Thrombogenicity of polysaccharidecoated surfaces［J］. Biomaterials, 2003, 24(11):1917-1924.
［16］Nishino T, Yokoyama G, Dobashi K, et al. Isolation, purification, and characterization of fucosecontaining sulfated polysaccharides from the brown seaweed Ecklonia kurome and their bloodanticoagulant activities ［J］. Carbohydr Res, 1989,186(1):119-148.
［17］Manju S, Muraleedharan CV. Evaluation of alginate dialdehyde crosslinked gelatin hydrogel as a biodegradable sealant for polyester vascular graft［J］. J Biomed Mater Res B Appl Biomater, 2011 ,98(1):139-188.
［18］王琴梅,张亦霞,李卓萍,等. 多醛基海藻酸钠交联剂的制备及性能［J］. 应用化学, 2010,27(2):155-158.
［19］Bouhadir KH, Lee KY, Alsberg E, et al. Degradation of partially oxidized alginate and its potential application for tissue engineering ［J］. Biotechnol Prog,2001 ,17(5):945-995.