Tissue Engineered Bone Constructed by Sintered Bone with Surface Mineralization Modification/BMP-2-Related Peptide and MC3T3-E1 Cells
1 Department of Orthopadics, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
2 Department of Orthopaedics,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China
Abstract:The aim of this study is to investigate the osteogenesis of tissue engineered bone constructed by sintered bone with surface mineralization modification/BMP2related peptide (P24) composite with MC3T3-E1 cells in vivo. The biomaterials were divided into three groups: Group A (sintered bone with surface mineralization modification / P24 composite); Group B (sintered bone with surface mineralization modification) and Group C (sintered bone). The MC3T3-E1 cells were induced by osteogenic medium and then seeded into the three materials. The cellmaterial samples were observed by inverted microscopy and environment scanning electron microscopy (ESEM). Eighteen New Zealand white rabbits were divided into three groups with 6 in each group. The model of sacrospinalis myobag was established by implanting above material samples in the myobag on the two sides. Every three rabbits in the three groups were killed at the 4thand 8th week after implantation, and then the new bone was observed by histologically (HE) and the areas of new osseous tissues were measured. Results from the inverted microscopy and ESEM showed that cells were grown well on the surface of the three materials, and BMP2 related peptide of Group A was confirmed to improve the adhesion rate and proliferation ability of MC3T3-E1 cells, and maintained the morphology of cells. At different postoperative time points, HE staining results showed new bone of Group A was significantly more than that of Group B and Group C, and that of Group B was significantly more than that of Group C. The areas of the new osseous tissue of group A, B, and C were (19 712.524±3 782.126) μm2,(28 227.617±2 455.375) μm2, and (11 648.507±1 047.221) μm2 at the 4thweek postsurgery, and (14 592.892±899.532)μm2, (7 986.655±903.487)μm2, and (11254.822±669.508) μm2 at 8th week postsurgery respectively. Sintered bone with surface mineralization modification / P24 composite is an ideal scaffold for bone tissue engineering, which is expected to be applied to clinical studies.
[1]Lane JM, Tomin E, Bostrom MPG. Biosynthetic Bone Grafting[J]. Clin Orthop, 1999, 367:S107-S117.
[2]Yaszemski MJ, Payne RG, Hayes WC, et al. Evolution of bone transplantation: molecular, cellular and tissue strategies to engineer human bone [J]. Biomaterials, 1996, 17:175-185.
[3]Chang SC, Chuang H, Chen YR, et al. Cranial repair using BMP2 gene engineered bone marrow stromal cells [J]. J Surg Res, 2004, 119: 85-91.
[4]Li Xiaoke, Chang Jiang. Preparation and characterization of bioactive collagen/ wollastonite composite scaffolds [J]. J Mater Sci Mater Med, 2005, 16:361-365.
[5]Hsu HC, Wu SC, Yang CH, et al. ZrO(2)/hydroxyapatite coating on titanium by electrolytic deposition[J]. J Mater Sci Mater Med, 2009, 20:615-619.
[6]Herliansyah MK, Hamdi M, IdeEktessabi A, et al. The influence of sintering temperature on the properties of compacted bovine hydroxyapatite[J]. Mater Sci Eng, 2009, 29: 1674-1680.
[7]李景峰,郑启新,郭晓东. 表面修饰煅烧骨的制备及其理化性质的研究[J].生物医学工程学杂志,2012,29(3):474-478.
[8]Wu Bin, Zheng Qixin, Guo Xiaodong, et al. Preparation and ectopic osteogenesis in vivo of scaffold based on mineralized recombinant humanlike collagen loaded with synthetic BMP2derived peptide [J]. Biomed Mater, 2008, 3:44111.
[9]Culpepper BK, Phipps MC, Bonvallet PP, et al. Enhancement of peptide coupling to hydroxyapatite and implant osseointegration through collagen mimetic peptide modified with a polyglutamate domain[J]. Biomaterials, 2010, 31(36): 9586-9594.
[10]Li Jingfeng, Hong Jijun, Zheng Qixin, et al. Repair of rat cranial bone defects with nHAC/PLLA and BMP2related peptide or rhBMP2[J]. J Orthop Res, 2011,29(11):1745-1752.
[11]郑启新, 刘苏南. 煅烧牛松质骨的制备、理化性能及生物相容性研究[J]. 生物医学工程学杂志, 2005, 22(1):95-98.
[12]Li Jingfeng, Lin Zhenyu, Zheng Qixin, et al. Repair of rabbit radial bone defects using true bone ceramics combined with BMP2related peptide and type I collagen[J]. Mater Sci Eng C, 2010, 30: 1272-1279.