Tissue Engineered Bone Constructed by Sintered Bone with Surface Mineralization Modification/BMP-2-Related Peptide and MC3T3-E1 Cells
1 Department of Orthopadics, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
2 Department of Orthopaedics，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China
Abstract：The aim of this study is to investigate the osteogenesis of tissue engineered bone constructed by sintered bone with surface mineralization modification/BMP2related peptide (P24) composite with MC3T3-E1 cells in vivo. The biomaterials were divided into three groups: Group A (sintered bone with surface mineralization modification / P24 composite); Group B (sintered bone with surface mineralization modification) and Group C (sintered bone). The MC3T3-E1 cells were induced by osteogenic medium and then seeded into the three materials. The cellmaterial samples were observed by inverted microscopy and environment scanning electron microscopy (ESEM). Eighteen New Zealand white rabbits were divided into three groups with 6 in each group. The model of sacrospinalis myobag was established by implanting above material samples in the myobag on the two sides. Every three rabbits in the three groups were killed at the 4thand 8th week after implantation, and then the new bone was observed by histologically (HE) and the areas of new osseous tissues were measured. Results from the inverted microscopy and ESEM showed that cells were grown well on the surface of the three materials, and BMP2 related peptide of Group A was confirmed to improve the adhesion rate and proliferation ability of MC3T3-E1 cells, and maintained the morphology of cells. At different postoperative time points, HE staining results showed new bone of Group A was significantly more than that of Group B and Group C, and that of Group B was significantly more than that of Group C. The areas of the new osseous tissue of group A, B, and C were (19 712.524±3 782.126) μm2,(28 227.617±2 455.375) μm2, and (11 648.507±1 047.221) μm2 at the 4thweek postsurgery, and (14 592.892±899.532）μm2, (7 986.655±903.487)μm2, and (11254.822±669.508) μm2 at 8th week postsurgery respectively. Sintered bone with surface mineralization modification / P24 composite is an ideal scaffold for bone tissue engineering, which is expected to be applied to clinical studies.
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