Research of β-TCP Bone Tissue Engineering Scaffolds Modified with Type I Collagen Based on Three-Dimensional Printing Technique
Sun Kaiyu1, Xu Mingen1,2*, Zhou Yongyong3
1 School of Life Information Instrument and Science Engineering, Hangzhou Dianzi University, Hangzhou 310018, China 2 Zhejiang Provincial Key Lab of Medical Information and Three-Dimensional Bio-Printing, Hangzhou 310018, China 3 Hangzhou Regenovo Biotechnology Co., Ltd., Hangzhou 310018, China
Abstract：Fabricating individualized tissue engineering scaffolds based on the three-dimensional shape of patient bone defects is required for the successful clinical application of bone tissue engineering.In this work, type I collagen gel was coated on individuated β-TCP scaffolds through 3D printing technique for bone repair.By comparing the influence of filling angle of 0/90°, 0/60°, 0/45°and concentration of coated collagen of 0.10 mg/mL, 0.25 mg/mL, 0.5 mg/mL on the pore diameter, porosity and mechanical properties of β-TCP scaffold, the β-TCP/ collagen scaffold with an optimal filling angle of 0/90° and an optimal concentration of 0.5 mg/mL for coated collagen was chosen, which was able to accurately reproduce the 3D model of design by equipping itself with multilevel pore structure whose mean diameter of megalopore and micropore were 315 μm and 3~5 μm respectively with a porosity of 84%. Meanwhile, due to the compression strength of 12.29±0.88 MPa and elasticity modulus of 116.74±27.75 MPa, it has quite a similarity with adult cancellous bone.In vitro culturing experiments of mouse bone marrow mesenchymal stem cells (mBMSCs)demonstrated that the coated collagen promoted the bioactivity and osteogenic properties, including better cytocompatibility, cell adhesion, proliferation, alkaline phosphatase (ALP) activity, and bone-related gene expressions (Collagen-I, BSP).The results showed that the collagen gel coated β-TCP scaffoldshad the matching shape,good controllable porosity and good osteogenic activity for mBMSCs through 3D printing technique.
孙开瑜,徐铭恩,周永勇. 基于3D打印的I型胶原涂覆β-TCP骨组织工程支架研究[J]. 中国生物医学工程学报, 2018, 37(3): 335-343.
Sun Kaiyu, Xu Mingen, Zhou Yongyong. Research of β-TCP Bone Tissue Engineering Scaffolds Modified with Type I Collagen Based on Three-Dimensional Printing Technique. Chinese Journal of Biomedical Engineering, 2018, 37(3): 335-343.
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