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Fabrication and Evaluation of Polyurethane Scaffolds with HoneycombLike Structure |
State Key Laboratory for Mechanical Behaviors of Materials, Xi′an Jiaotong University, Xi′an 710049, China
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Abstract In this study, poly (εcaprolactone) diol was synthesized via ringopening polymerization of εcaprolactone using 2,2′dithiodiethanol as an initiator, and then polyurethane was synthesized through addition polymerization of poly (εcaprolactone) and hexamethylene diisocyanate. Polyurethane was characterized by FTIR, XRD and DSC. Polyurethane scaffolds were fabricated by biotemplating technique, and their porous structure, porosity and mechanical properties were characterized by SEM, archimedes principle based method and mechanical test, respectively. MC3T3E1 cells were cultured on polyurethane scaffolds to evaluate their cytocompatibility. The results indicated that polyurethane scaffolds had a honeycomblike channels with a diameter range of 100 μm to 140 μm, and their porosity and compressive modulus were 772%±41% and 29902 kPa, respectively. Immunofluorescence staining and live/dead staining results indicated that MC3T3E1 cells could adhere and proliferate on the scaffolds. The results suggested that the honeycomblike polyurethane scaffolds were a promising candidate for bone tissue engineering.
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