C6位氧化型细菌纤维素/壳聚糖复合材料的制备及表征

doi:10.3969/j.issn.0258-8021. 2014. 05.011

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Abstract Bacterial cellulose (BC) is a natural scaffold for bone tissue engineering. To improve its degradability and microstructural controllability, C6oxidated bacterial cellulose/chitosan (OBC/CH) composite scaffolds were prepared by lyophilization of a mixture of OBC suspension and CH solution in a cylindrical mold. The original BC was subjected to 2,2,6,6tetramethylpyperidine1oxyl radical (TEMPO)/NaBr/NaClOmediated oxidation to introduce surface carboxyl groups before mixing. The integration of CH within OBC was performed by 1ethyl3(3dimethylaminopropyl) carbodiimide/Nhydroxysuccinimide (EDC/NHS)mediated crosslinking. The products were characterized by FTIR, solidstate 13CNMR, XRD, in vitro degradation, porosity and FESEM. The FTIR and 13CNMR results show that OBC and CH were crosslinked successfully; XRD result shows that the crystallinity of the products was slightly decreased with the increase of CH content, the difference was less than 31%; the degradability of OBC and OBCCH in vitro was increased significantly, and the degradation rate within 8 weeks was 402% when ((OBCCOOH)/(CHNH2))/(n/n)＝1/45; FESEM and porosity results show that the pore size and porosity of OBCCH scaffolds were greater than that of BC and OBC scaffolds, and the maximum porosity was reached 97% when ((OBCCOOH)/(CH0NH2))/(n/n)＝1/45. In conclusion, OBCCH is an ideal scaffold for bone tissue engineering, with good degradability and microstructural controllability.

Key words： bacterial cellulose TEMPO chitosan composite

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	LIAO ShiBo¹
	2HUANG Shu Yu²LAI Chen¹XI TingFei^1#*WU Min^2LIU Yu Ping²YAN Yi Min²

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LIAO ShiBo^1,2HUANG Shu Yu²LAI Chen¹XI TingFei^1#*WU Min^2LIU Yu Ping²YAN Yi Min². Preparation and Characterization of C6Oxidated Bacterial Cellulose/Chitosan Composite[J]. journal1, 2014, 33(5): 600-608.

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http://cjbme.csbme.org/EN/ 10.3969/j.issn.0258-8021. 2014. 05.011 OR http://cjbme.csbme.org/EN/Y2014/V33/I5/600

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