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Abstract More and more scientists focus on the growth factors that can improve the bone regeneration. The aim of the study was to prepare a bone scaffolds, and to investigate underlying molecular mechanisms in promoting osteogenesis on the socket of tooth extraction. Chitosan microspheres carrying two osteogenic factors, BMP2 or APN, were prepared by emulsion crosslinking, and then were mixed with ceramic bovine bone to form a novel drug delivery system. To compare the biological activity of the grafts, MC3T3E1 cells were seeded in the scaffold. The influence of the scaffold on cells proliferation and differentiation were investigated. Results show that the average diameter of the chitosan microspheres is 9634 μm. The scaffolds loading BMP2 or APN both contributed to the proliferation of osteoblasts MC3T3E1. Cells seeded in the scaffold loading BMP2 showed higher proliferation. However, cells seeded in the adiponectinloading scaffold showed a higher level in the expression of osteogenesisrelated proteins and genes compared with those in the BMP2 loading scaffold.
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［1］Araujo MG, Lindhe J. Dimensional ridge alterations following tooth extraction. an experimental study in the dog ［J］. J Clin Periodontol, 2005, 32(2):212-218. ［2］Araujo M. The influence of biooss collagen on healing of an extraction socket: an experimental study in the dog ［J］. Int J Periodontics Restorative Dent, 2008, 28(2): 123-135.
［3］Carmagnola D. Healing of human extraction sockets filled with BioOss ［J］. Clin Oral Implants Res, 2003, 12(2):137-143.
［4］Bashara H. The effect of permanent grafting materials on the preservation of the buccal bone plate after tooth extraction: an experimental study in the dog ［J］. Clin Oral Implants Res, 2012, 23(8): 911-917.
［5］Shi B. Alveolar ridge preservation prior to implant placement with surgicalgrade calcium sulfate and plateletrich plasma: a pilot study in a canine model ［J］. Int J Oral Maxillofac Implants, 2007, 22(4): 656-665.
［6］LissenbergThunnissen SN, Gorter DJ, Sier CF, et al. Use and efficacy of bone morphogenetic proteins in fracture healing ［J］. Int Orthop, 2011, 35(9): 1271-1280.
［7］Matin K. Impact of recombinant human bone morphogenetic protein2 on residual ridge resorption after tooth extraction: an experimental study in the rat ［J］. Int J Oral Maxillofac Implants, 2001, 16(3): 400-411.
［8］Spagnoli DB, Marx RE. Dental implants and the use of rhBMP2 ［J］. Oral Maxillofac Surg Clin North Am, 2001, 232):347-361.
［9］Katagiri T, Takahashi N. Regulatory mechanisms of osteoblast and osteoclast differentiation ［J］. Oral Dis, 2002, 8(3): 147-159.
［10］Martin TJ, Sims NA. Osteoclastderived activity in the coupling of bone formation to resorption ［J］. Trends Mol Med, 2005, 11(2): 76-81.
［11］Yamauchi T. Cloning of adiponectin receptors that mediate antidiabetic metabolic effects ［J］. Nature, 2003,423(6941): 762-769.
［12］Diez JJ, Iglesias P. The role of the novel adipocytederived hormone adiponectin in human disease ［J］. Eur J Endocrinol, 2003, 148(3): 293-300.
［13］Deepa SS, Dong LQ. APPL1: role in adiponectin signaling and beyond ［J］.Am J Physiol Endocrinol Metab, 2009, 296(1): 22-36.
［14］Huang CY. Adiponectin increases BMP2 expression in osteoblasts via AdipoR receptor signaling pathway ［J］. J Cell Physiol, 2010, 224(2): 475-483.
［15］Tu Q. Adiponectin inhibits osteoclastogenesis and bone resorption via APPL1-mediated suppression of AKT1 ［J］. J Biol Chem, 2011, 286(14):12542-12553.
［16］Spagnoli DB, Marx RE. Dental implants and the use of rhBMP-2 ［J］. Oral Maxillofac Surg Clin North Am, 2011, 23(2):347-361.
［17］Katagiri, T, Takahashi N. Regulatory mechanisms of osteoblast and osteoclast differentiation ［J］.Oral Dis,2002, 8(3): 147-159.
［18］Martin TJ, Sims NA. Osteoclastderived activity in the coupling of bone formation to resorption ［J］. Trends Mol Med, 2005, 11(2): 76-81.
［19］Raisz, LG. Physiology and pathophysiology of bone remodeling ［J］. Clin Chem, 1999, 45(8 Pt 2): 1353-1358.
［20］Yamauchi T. Cloning of adiponectin receptors that mediate antidiabetic metabolic effects ［J］. Nature,2003,423(6941): 762-769.
［21］Makoto K, Hiroko K, Kenichirou K, et al. Promotion of tracheal cartilage growth by intratracheal injection of basic fibroblast growth factor (bFGF) ［J］. J Pediatr Surg,2014, 4(2):296-300.
［22］Aleksandar K, Ulrike H, Patricia M, et al. LIMhomeobox gene 2 promotes tumor growth and metastasis by inducing autocrine and paracrine PDGFB signaling ［J］. Mol Oncol,2014, 8(2):401-416.
［23］Tracey H, Brent LA. Comparison of cell viability on anorganic bone matrix with or without P15 cell binding peptide ［J］. Biomaterials, 2004, 25(19): 4831-4836.
［24］Shin K, Takashi S, Yoshio K, et al. Automatic construction of an anatomical coordinate system for threedimensional bone models of the lower extremitiesPelvis, femur, and tibia ［J］. J Biomech, 2014, 47(5): 1229-1233.
［25］Liao W, Yang S, Song C, et al. Construction of ureteral grafts by seeding bone marrow mesenchymal stem cells and smooth muscle cells into bladder a cellular matrix ［J］. Trasplantation Proc, 2013, 45(2): 730-734. |
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