蚕丝蛋白/PCL共混取向纤维的力学性质与细胞响应

doi:10.3969/j.issn.0258-8021.2015.03.011

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Abstract Due to its mechanical strength, biocompatibility and sterilizability, silk fibroin is frequently utilized as a natural boneregeneration material. To improve its mechanical character and reinforce cell movement induction, a highly aligned electrospinning fibrous scaffold of PCL/silk fibroin blend was fabricated with a rotating collector. Scanning electron microscope images were analyzed and mechanical properties of the scaffold were investigated by tensile mechanical tests, and compared to nonoriented scaffolds. Furthermore, mesenchymal stem cells were planted on the scaffolds to investigate its biocompatibility and cell movement insitu. Results shows that 91% fibers on the aligned fibroin scaffold were distributed between the dominant direction +/-10°. With ideal support for stem cell proliferation, the aligned fibrous scaffold could also induce cell elongation at a length of 23646±82 μm and distribution along the dominant fiber direction with a narrow spreading angle at 657±445 °. In conclusion, this novel aligned fibroin scaffold could provide a moderate mesenchymal stem cell engraftment interface and speedup the early stage cell movement toward the bone defect.

Key words： electrospinning silk fibroin mesenchymal stem cell aligned fibrous scaffold cell movement

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http://cjbme.csbme.org/EN/ 10.3969/j.issn.0258-8021.2015.03.011 OR http://cjbme.csbme.org/EN/Y2015/V34/I3/337

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