交联对氧化纤维素/蚕丝蛋白复合膜结构和性能的影响

doi:10.3969/j.issn.0258-8021.2015. 02.012

Abstract
Figure/Table
References (0)
Related Citation (7)

Download: PDF (5563 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract The purpose of this study is to investigate two kinds of preparation methods on nanobacterial cellulose/silk fibroin composites and to compare the physical and chemical properties of the resulting composite products. One method is to mix directly bacterial cellulose or oxidized bacterial cellulose membranes with silk fibroin solution. Another one is to add crosslinking agents during the mixing process. The composite products were characterized using Fourier transforminfrared (FTIR) spectroscopy,13C NMR spectrum, Xray photoelectron spectroscopy (XPS), field emission scanning electron microscopy (FESEM) and mechanical testing. Results show that fibroin can successfully be combined on the oxidized bacterial cellulose membrane, XPS results show that no matter adding crosslinking agents or not, C1s, O1s and N1s appeared no difference. BC/SF composite membrane, the comparison C/N molar ratio of without crosslinking agents and with them, reduced from 963 to 394, while the TBC/SF, C/N ratio increased from 503 to 741. The membrane surface of one group containing crosslinking agents is smoother than another, and the structure has obvious changes. There are significant differences in elongation at break of TBC/SF (P＜001). Based on this work, the performance of composite films prepared by adding crosslinking agents is better, and the materials described here demonstrate the potential in medical material applications, especially in a cellular vascular graft filed.

Key words： bacterial cellulose oxidation silk fibroin composite

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	Wang Shuaiyan¹Huang Tao¹Lai Chen² Xi Tingfei¹
	2#*Nan Fang¹

Cite this article:

Wang Shuaiyan¹Huang Tao¹Lai Chen² Xi Tingfei^1,2#*Nan Fang¹. The Effect of Crosslink on the Structures and Performance of Oxidized Bacterial Cellulose/Silk Fibroin Composite Films[J]. journal1, 2015, 34(2): 212-220.

URL:

http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2015. 02.012 OR http://cjbme.csbme.org/EN/Y2015/V34/I2/212

［1］Torres FG, Commeaux S, Troncoso OP, et al. Biocompatibility of bacterial cellulose based biomaterials ［J］. Biomaterials, 2012, 3(4): 864-878. 
［2］Petersen N, Gatenholm P. Bacterial cellulosebased materials and medical devices: current state and perspectives. Appl Microbiol Biotechnol, 2011, 91(5): 1277-1286. 
［3］Fitz-Binder C, Bechtold T. Onesided surface modification of cellulose fabric by printing a modified TEMPOmediated oxidant ［J］. Carbohydr Polym, 2014, 106: 142-147.
［4］Retegi A, Algar I, Martin L, et al. Sustainable optically transparent composites based on epoxidized soybean oil (ESO) matrix and high contents of bacterial cellulose (BC) ［J］. Cellulose, 2012, 19: 103-109.
［5］Wiegand C, Elsner P, Hiple UC. Protease and ROS activities influenced by a composite of bacterial cellulose and collagen type I in vitro ［J］. Cellulose, 2006, 13 (6): 689-696. 
［6］Ciechanska D. Multifunctional bacterial cellulose/chitosan composite materials for medical applications ［J］. Fibres and Textiles, 2004, 12(4): 69-72
［7］DieterA, Schumann JW, Dieter O, et al. Artificial vascular implants from bacterial cellulose: preliminary results of small arterial substitutes［J］. Cellulose, 2009, 16:877-885.［8］Malm CJ, Risberg B, Bodin A, et al. Small calitre biosynthetic cellulose blood vessels:13months patercy in a sheep model ［J］. Scandonavian Cardiovascular Journal, 2012,46(1): 57-62. 
［9］Hutchens SA, Benson RS, Evans BR, et al. Biomimetic synthesis of calciumdeficient hydroxyapatite in a natural hydrogel ［J］. Biomaterials, 2006, 27(26): 4 661-4670.
［10］Bodin A，Bharadwaj S, Wu S, et al. Tissue engineered conduit using urinederived stem cells seeded bacterial cellulose polymer in urinary reconstruction and diversion ［J］. Biomaterials，2010, 31(34): 8889-8901. 
［11］Svensson A, Nicklasson E, Harrah T, et al. Cellulose as a potential scaffold for tissue engineering of cartilage ［J］. Biomaterials, 2005, 26: 419-431.
［12］Saito T, Isogai A. TEMPOmediated oxidation of native cellulose: The effect of oxidation conditions on chemical and crystal structures of the waterinsoluble fractions ［J］. Biomacromolecules, 2004, 5: 1983-1989.
［13］Saito T, Shibata I, Isogai A, et al. Distribution of carboxylate groups introduced into cotton linters by the TEMPOmediated oxidation ［J］. Biomacromolecules, 2005, 61: 414-419. 
［14］Saito T, Okita Y, Nge TT, et al. EMPOmediated oxidation of native cellulose: Microscopic analysis of fibrous fractions in the oxidized products ［J］. Carbohydrate Polymers, 2006, 65(4): 435-440.
［15］Kowalska-Ludwicka K, Cala J, Grobelski B, et al. Modified bacterial cellulose tubes for regeneration of damaged peripheral nerves ［J］. Arch Med Sci, 2013, 9(3): 527-534.
［16］Inouye K, Kurokawa M, Nishikawa S, et al. Use of Bombyx mori silk fibroin as a substratum for cultivation of animal cells ［J］. J Biochem Biophys Meth, 1998, 37(3): 159-164. 
［17］Irene C, Marina F, Nadia A, et al. In vivo regeneration of elastic lamina on fibroin biodegradable vascular scaffold ［J］. Int J Artif Organs, 2013, 36(3): 166-174. 
［18］Yang Xiaoyuan, Wang Lu, Guan Guoping, et al. Preparation and evaluation of biocomponent and homogeneous polyester silk small diameter arterial prostheses ［J］. Journal of Biomaterials Applications, 2012, 27(7): 1-12.
［19］Losi P, Lombardi S, Briganti E, et al. Luminal surface microgeometry affects platelet adhesion in smalldiameter synthetic grafts ［J］. Biomaterials, 2004, 25(18): 4447-4455.
［20］Robinson WP, Owens CD, Nguyen LL, et al. Inferior outcomes of autogenous infrainguinal bypass in Hispanics: An analysis of ethnicity, graft function, and limb salvage ［J］. J Vasc Surg, 2009, 49 (6): 1416-1425.
［21］Madhavan K, Belchenko D, Motta A, et al. Evaluation of composition and crosslinking effects on collagenbased composite constructs ［J］. Acta Biomater, 2010, 6 (4) : 1413-1422. 
［22］Liimatainen H, Visanko M, Sirvi JA, et al. Enhancement of the nanofibrillation of wood cellulose through sequential periodatechlorite oxidation ［J］. Biomacromolecules, 2012, 13: 1592-1597. 
［23］Freddi G, Romano M, Rosaria MJ. Silk fibroin/cellulose blend films: Preparation, structure, and physical properties ［J］. Appl Polym Sci, 1995, 56(12): 1537-1545. 
［24］Shang Songmin, Zhu Lei, Fan Jintu. Physical properties of silk fibroin/cellulose blend films regenerated from the hydrophilic ionic liquid ［J］. Carbohydr Polym, 2011, 86(2): 462-468.
［25］Jung R, Jin HJ. preparation of silk fibroin/bacterial cellulose composite films and their mechanical properties ［J］. Key Engineering Materials, 2007, 342-343: 741-744.
［26］Klemm D, Philpp B, Heinze T, et al. Comprehensive Cellulose Chemistry: Fundamentals and Analytical Methods, Volume 1 ［M］. Weinheim: WileyVCH, 1998.
［27］Vitor RJ, Newman RH, Ha MA, et al. Conformational features of crystalsurface cellulose from higher plants ［J］. Plant J, 2002, 30(6): 721-731.
［28］Asakura T, Kuzuhara A, Tabeta R, et al. Conformational characterization of Bombyx mori silk fibroin in the solid state by highfrequency carbon13 cross polarizationmagic angle spinning NMR, xray diffraction, and infrared spectroscopy ［J］. Macromolecules, 1985, 18(10): 1841-1845.
［29］Wang M, Yuan J, Huang XB, et al. Grafting of carboxybetaine brush onto cellulose membranes via surfaceinitiated ARGETATRP for improving blood compatibility ［J］. Colloids and Surfaces B: Biointerfaces, 2013, 103: 52-58.
［30］Lin H, Yao LR, Chen YY, et al. Structure and properties of silk fibroin modified cotton ［J］. Fiber Polym, 2008, 9(2): 113-120.
［31］Lai Chen, Sheng Liyuan, Liao Shibo, et al. Surface characterization of TEMPOoxidized bacterial cellulose ［J］. Interface Anal, 2013, 45: 1673-1679.
［32］Brown EE, Zhang Jinwen, Laborie MG. Neverdried bacterial cellulose/fibrin composites: preparation, morphology and mechanical properties ［J］. Cellulose, 2011, 18: 631-641.