外泌体及其在组织工程中的应用

doi:10.3969/j.issn.0258-8021.2020.01.14

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Abstract Mesenchymal stem cells are adult stem cells with self-renewal ability, which are widely found in tissues such as bone marrow, adipose tissue, and peripheral blood. Mesenchymal stem cells have many therapeutic effects in the clinic currently, which brings hope for the cure of many diseases. Recent studies have shown that mesenchymal stem cells release many kinds of small molecular substances, among them, exosomes carrying large amounts of biologically active factors can mediate communication between cells and play a role in physiological and pathological processes. As a research hotspot of cell-free tissue engineering in recent years, exosomes have contributed to solutions in clinical applications of regenerative medicine to some extent, such as seed cell source, quantity and immune rejection, and at the same time in disease diagnosis and treatment. This paper introduces the methods of exosome extraction and identification and its potential application in tissue engineering and discusses its future development.

Key words： exosomes extraction identification applications tissue engineering

Received: 16 April 2019

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R318

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	Articles by authors
	Liu Yufei
	Zhang Zhiling
	Wang Yanying
	Dong Yunsheng
	Zhang Lin
	Wang Shufang

Cite this article:

Liu Yufei,Zhang Zhiling,Wang Yanying, et al. Exosomes and Their Applications in Tissue Engineering[J]. Chinese Journal of Biomedical Engineering, 2020, 39(1): 109-113.

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2020.01.14 OR http://cjbme.csbme.org/EN/Y2020/V39/I1/109

[1] Liang X, Ding Y, Zhang Y, et al. Paracrine mechanisms of mesenchymal stem cell-based therapy: Current status and perspectives [J]. Cell Transplant, 2014,23(9):1045-1059.
[2] Song M, Heo J, Chun JY, et al. The paracrine effects of mesenchymal stem cells stimulate the regeneration capacity of endogenous stem cells in the repair of a bladder-outlet-obstruction-induced overactive bladder [J]. Stem Cells Dev, 2014,23(6):654-663.
[3] Ratajczak MZ, Kucia M, Jadczyk T, et al. Pivotal role of paracrine effects in stem cell therapies in regenerative medicine: Can we translate stem cell-secreted paracrine factors and microvesicles into better therapeutic strategies? [J]. Leukemia, 2012,26(6):1166-1173.
[4] Johnstone RM, Adam M, Hammond JR, et al. Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes) [J]. J Biol Chem, 1987, (19):9412-9420.
[5] Raposo G, Nijman HW, Stoorvogel W, et al. B lymphocytes secrete antigen-presenting vesicles [J]. J Exp Med, 1996,183(3):1161-1172.
[6] Anderson HC. Vesicles associated with calcification in the matrix of epiphyseal cartilage [J]. J Cell Biol, 1969,41(1):59-72.
[7] Crawford N. The presence of contractile proteins in platelet microparticles isolated from human and animal platelet-free plasma [J]. Br J Haematol, 1971,21(1):53-69.
[8] Stegmayr B, Ronquist G. Promotive effect on human sperm progressive motility by prostasomes [J]. Urol Res, 1982,10(5):253-257.
[9] Trams EG, Lauter CJ, Salem N, Jr., et al. Exfoliation of membrane ecto-enzymes in the form of micro-vesicles [J]. Biochim Biophys Acta, 1981,645(1):63-70.
[10] Dvorak HF, Quay SC, Orenstein NS, et al. Tumor shedding and coagulation [J]. Science, 1981,212(4497):923-924.
[11] George JN, Thoi LL, McManus LM, et al. Isolation of human platelet membrane microparticles from plasma and serum [J]. Blood, 1982,60(4):834-840.
[12] Thery C, Amigorena S, Raposo G, et al. Isolation and characterization of exosomes from cell culture supernatants and biological fluids [J]. Curr Protoc Cell Biol, 2006,Chapter 3:Unit 3.22.
[13] Baietti MF, Zhang Z, Mortier E, et al. Syndecan-syntenin-alix regulates the biogenesis of exosomes [J]. Nat Cell Biol, 2012, 14(7):677-685.
[14] Taylor DD, Chou IN, Black PH. Isolation of plasma membrane fragments from cultured murine melanoma cells [J]. Biochem Biophys Res Commun, 1983,113(2):470-476.
[15] Escola JM, Kleijmeer MJ, Stoorvogel W, et al. Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human b-lymphocytes [J]. J Biol Chem, 1998,273(32):20121-20127.
[16] Lamparski HG, Metha-Damani A, Yao JY, et al. Production and characterization of clinical grade exosomes derived from dendritic cells [J]. J Immunol Methods, 2002,270(2):211-226.
[17] Conde-Vancells J, Rodriguez-Suarez E, Embade N, et al. Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes [J]. J Proteome Res, 2008,7(12):5157-5166.
[18] Raposo G, Stoorvogel W. Extracellular vesicles: Exosomes, microvesicles, and friends [J]. J Cell Biol, 2013,200(4):373-383.
[19] Chen L, Chen R, Kemper S, et al. Pathways of production and delivery of hepatocyte exosomes [J]. J Cell Commun Signal, 2018,12(1):343-357.
[20] Sitar S, Kejzar A, Pahovnik D, et al. Size characterization and quantification of exosomes by asymmetrical-flow field-flow fractionation [J]. Anal Chem, 2015,87(18):9225-9233.
[21] Ma J, Zhao Y, Sun L, et al. Exosomes derived from akt-modified human umbilical cord mesenchymal stem cells improve cardiac regeneration and promote angiogenesis via activating platelet-derived growth factor d [J]. Stem Cells Transl Med, 2017,6(1):51-59.
[22] Sahoo S, Losordo DW. Exosomes and cardiac repair after myocardial infarction [J]. Circ Res, 2014,114(2):333-344.
[23] Zhang B, Wang M, Gong A, et al. Hucmsc-exosome mediated-wnt4 signaling is required for cutaneous wound healing [J]. Stem Cells, 2015,33(7):2158-2168.
[24] Zhang B, Wu X, Zhang X, et al. Human umbilical cord mesenchymal stem cell exosomes enhance angiogenesis through the wnt4/beta-catenin pathway [J]. Stem Cells Transl Med, 2015,4(5):513-522.
[25] Bruno S, Grange C, Deregibus MC, et al. Mesenchymal stem cell-derived microvesicles protect against acute tubular injury [J]. J Am Soc Nephrol, 2009,20(5):1053-1067.
[26] Drommelschmidt K, Serdar M, Bendix I, et al. Mesenchymal stem cell-derived extracellular vesicles ameliorate inflammation-induced preterm brain injury [J]. Brain Behav Immun, 2017,60:220-232.
[27] Lee C, Mitsialis SA, Aslam M, et al. Exosomes mediate the cytoprotective action of mesenchymal stromal cells on hypoxia-induced pulmonary hypertension [J]. Circulation, 2012,126(22):2601-2611.
[28] Ti D, Hao H, Tong C, et al. Lps-preconditioned mesenchymal stromal cells modify macrophage polarization for resolution of chronic inflammation via exosome-shuttled let-7b [J]. J Transl Med, 2015,13:308.
[29] Huey DJ, Hu JC, Athanasiou KA. Unlike bone, cartilage regeneration remains elusive [J]. Science, 2012,338(6109):917-921.
[30] Klein J. Chemistry. Repair or replacement--A joint perspective [J]. Science, 2009,323(5910):47-48.
[31] Hu GW, Li Q, Niu X, et al. Exosomes secreted by human-induced pluripotent stem cell-derived mesenchymal stem cells attenuate limb ischemia by promoting angiogenesis in mice [J]. Stem Cell Res Ther, 2015,6(1):10.
[32] Liu X, Li Q, Niu X, et al. Exosomes secreted from human-induced pluripotent stem cell-derived mesenchymal stem cells prevent osteonecrosis of the femoral head by promoting angiogenesis [J]. Int J Biol Sci, 2017,13(2):232-244.
[33] Zhang J, Liu X, Li H, et al. Exosomes/tricalcium phosphate combination scaffolds can enhance bone regeneration by activating the pi3k/akt signaling pathway [J]. Stem Cell Res Ther, 2016,7(1):136.
[34] Furuta T, Miyaki S, Ishitobi H, et al. Mesenchymal stem cell-derived exosomes promote fracture healing in a mouse model [J]. Stem Cells Transl Med, 2016,5(12):1620-1630.
[35] Qi X, Zhang J, Yuan H, et al. Exosomes secreted by human-induced pluripotent stem cell-derived mesenchymal stem cells repair critical-sized bone defects through enhanced angiogenesis and osteogenesis in osteoporotic rats [J]. Int J Biol Sci, 2016,12(7):836-849.
[36] Zhang S, Teo KYW, Chuah SJ, et al. Msc exosomes alleviate temporomandibular joint osteoarthritis by attenuating inflammation and restoring matrix homeostasis [J]. Biomaterials, 2019,200:35-47.
[37] Liu X, Yang Y, Li Y, et al. Integration of stem cell-derived exosomes with in situ hydrogel glue as a promising tissue patch for articular cartilage regeneration [J]. Nanoscale, 2017,9(13):4430-4438.
[38] Bianco P, Cao X, Frenette PS, et al. The meaning, the sense and the significance: Translating the science of mesenchymal stem cells into medicine [J]. Nat Med, 2013,19(1):35-42.
[39] Fennema EM, Tchang LAH, Yuan H, et al. Ectopic bone formation by aggregated mesenchymal stem cells from bone marrow and adipose tissue: A comparative study [J]. J Tissue Eng Regen Med, 2018,12(1):e150-e158.
[40] Li W, Liu Y, Zhang P, et al. Tissue-engineered bone immobilized with human adipose stem cells-derived exosomes promotes bone regeneration [J]. ACS Appl Mater Interfaces, 2018,10(6):5240-5254.
[41] Liu T, Liu S, Zhang K, et al. Endothelialization of implanted cardiovascular biomaterial surfaces: The development from in vitro to in vivo [J]. J Biomed Mater Res A, 2014,102(10):3754-3772.
[42] Filova E, Straka F, Mirejovsky T, et al. Tissue-engineered heart valves [J]. Physiol Res, 2009,58(Suppl 2):S141-S158.
[43] Oh BH, Kaligis RW, Wang Y, et al. Survey of atherosclerotic disease in asian subjects with cardiovascular disease risk factors who were not receiving lipid-lowering agents [J]. Int J Cardiol, 2013,168(3):2761-2766.
[44] Rocco KA, Maxfield MW, Best CA, et al. In vivo applications of electrospun tissue-engineered vascular grafts: A review [J]. Tissue Eng Part B Rev, 2014,20(6):628-640.
[45] Du W, Zhang K, Zhang S, et al. Enhanced proangiogenic potential of mesenchymal stem cell-derived exosomes stimulated by a nitric oxide releasing polymer [J]. Biomaterials, 2017,133:70-81.
[46] Wei Y, Wu Y, Zhao R, et al. Msc-derived sevs enhance patency and inhibit calcification of synthetic vascular grafts by immunomodulation in a rat model of hyperlipidemia [J]. Biomaterials, 2019,204:13-24.
[47] Han Y, Ren J, Bai Y, et al. Exosomes from hypoxia-treated human adipose-derived mesenchymal stem cells enhance angiogenesis through vegf/vegf-r [J]. Int J Biochem Cell Biol, 2019,109:59-68.
[48] Wang C, Wang M, Xu T, et al. Engineering bioactive self-healing antibacterial exosomes hydrogel for promoting chronic diabetic wound healing and complete skin regeneration [J]. Theranostics, 2019,9(1):65-76.
[49] Ma T, Fu B, Yang X, et al. Adipose mesenchymal stem cell-derived exosomes promote cell proliferation, migration, and inhibit cell apoptosis via wnt/beta-catenin signaling in cutaneous wound healing [J]. J Cell Biochem, 2019,120(6):10847-10854.
[50] Zhao B, Zhang Y, Han S, et al. Exosomes derived from human amniotic epithelial cells accelerate wound healing and inhibit scar formation [J]. J Mol Histol, 2017,48(2):121-132.
[51] Armstrong JP, Holme MN, Stevens MM. Re-engineering extracellular vesicles as smart nanoscale therapeutics [J]. ACS Nano, 2017,11(1):69-83.
[52] Wood MJ, O′Loughlin AJ, Samira L. Exosomes and the blood-brain barrier: Implications for neurological diseases [J]. Ther Deliv, 2011,2(9):1095-1099.