微囊泡产生及鉴定体系现状分析

doi:10.3969/j.issn.0258-8021.2020.03.14

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Abstract In recent years, with microvesicles (MV) studies development, how to effectively obtain a sufficient amount of high purity microvesicles has become a big challenge in this field. Microvesicles also known as microparticles, are small vesicles that fall off from the cell membrane after cell activation, injury or apoptosis. MV with a diameter of about 100～1000 nm can interact with cells through different mechanisms and play a similar biological function as the source cells do. At the same time, MV have become a new favorite in bionic drug carrier research because of their natural membrane components and good biological transmissibility. In this paper, we summarized the principle, application and key technologies, and the existing key technologies for extraction and identification of MV, and also made prospections about MV's application in the future.

Key words： microvesicles (MVs) extraction identification, medical application

Received: 09 October 2019

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R318

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2020.03.14 OR http://cjbme.csbme.org/EN/Y2020/V39/I3/362

[1] Raposo G, Stoorvogel W. Extracellular vesicles: Exosomes, microvesicles, and friends[J]. J Cell Biol, 2013, 200(4):373-383.
[2] Wolf P. The nature and significance of platelet products in human plasma[J].Br J Haematol, 1967, 13(3):269-288.
[3] Boulanger CM, Loyer X, Rautou PE. Extracellular vesicles in coronary artery disease[J].Nature Reviews Cardiology, 2017, 14:259-272.
[4] Cobelli NJ, Leong DJ, Sun HB. Exosomes: Biology, therapeutic potential, and emerging role in musculoskeletal repair and regeneration [J].Ann N Y Acad Sci, 2017, 1410(1): 57-67.
[5] Cocucci E, Racchetti G, Meldolesi J. Shedding microvesicles: Artefactsno more [J]. Trends Cell Biol, 2009, 19(2): 43-51.
[6] Quesenberry PJ, Goldberg LR, Aliotta JM, et al. Cellular phenotype and extracellular vesicles: Basic and clinical considerations [J]. Stem Cells Dev, 2014, 23(13): 1429-1436.
[7] Svedman FC, Lohcharoenkal W, Bottai M, et al. Extracellular microvesicle microRNAs as predictive biomarkers for targeted therapy in metastastic cutaneous malignant melanoma[J/OL].https://doi.org/10.1371/journal.pone.0206942, 2018-11-06/2019-10-09.
[8] Bavisotto CC, Scalia F, Gammazza AM, et al. Extracellular vesicle-mediated cell-cell communication in the nervous system: Focus on neurological diseases[J/OL].https://doi.org/10.3390/ijms20020434, 2019-01-20/2019-10-09.
[9] Jacopo B, Silvia M, Chiara G, et al. Stem cell-derived extracellular vesicles and immune-modulation[J/OL].www.frontiersin.org, 2016-08-22/2019-10-09.
[10] Taylor J, Bebawy M. Proteins regulating microvesicle biogenesis and multidrug resistance in cancer[J/OL].www.proteomics-journal.com, 2019-01-24/2019-10-09.
[11] Kim OY, Lee J, Gho YS.Extracellular vesicle mimetics: Novel alternatives to extracellular vesicle-based theranostics, drug delivery, and vaccines[J].Seminars in Cell & Developmental Biology, 2017, 67:74-82.
[12] Christopher T, James C, Crislyn DS.Biology and biogenesis of shed microvesicles[J].Small Gtpases, 2017, 8(4):220-232.
[13] Lee Y, Andaloussi SEL, Wood MJ.A.Exosomes and microvesicles: Extracellular vesicles for genetic information transfer and gene therapy[J].Human Molecular Genetics, 2012, 21(1):125-134.
[14] Xiao J, Cretoiu S. Exosomes in Cardiovascular Diseases[M]. Singapore:Springer Nature, 2017:10-26.
[15] Coutts AS, Weston L. Cell Cycle Oscillators: Methods and Protocols, Methods in Molecular Biology[M]. New York:Humana Press, 2016:91-99.
[16] Tong Jinjin, Sun Dongdong, Yang Chao, et al.Serum starvation and thymidine double blocking achieved efficient cell cycle synchronization and altered the expression of p27, p53, bcl-2 in canine breast cancer cells.[J].Research in Veterinary Science, 2016, 105:4-14.
[17] Francois B, Chan B, Antonyak MA, et al.Microvesicles released from tumor cells disrupt epithelial cell morphology and contractility[J].J Biomech, 2016, 49(8):1272-1279.
[18] Zhao Xiaoping, Wang Meng, Song Yong, et al.Membrane microvesicles as mediators for melanoma-broblasts communication: Roles of the VCAM-1/VLA-4 axis and the ERK1/2 signal pathway[J].Cancer Letters, 2015, 360(2):125-133.
[19] Xiao Huaitong, Feng Yuanyong, Tao Yueqin, et al.Microvesicles releasing by oral cancer cells enhance endothelial cell angiogenesis via Shh/RhoA signaling pathway[J].Cancer Biology & Therapy, 2017, 18(10):783-791.
[20] Milani G, Lana T, Bresolin S, et al. Expression profiling of circulating microvesicles reveals intercellular transmission of oncogenic pathways[J/OL].mcr.aacrjournals.org, 2017-02-15/2019-10-09.
[21] Ji Yuan, Ma Yongbin, Chen Xiang, et al.Microvesicles released from human embryonic stem cell derived-mesenchymal stem cells inhibit proliferation ofleukemia cells[J].Oncology Reports, 2017, 38:1013-1020.
[22] He W, Calore F, Londhe P, et al.Microvesicles containing miRNAs promote muscle cell death in cancer cachexia via TLR7[J]. PNAS, 2014, 111(12):4525-4529.
[23] Ke Tang, Yi Zhang, Huafeng Zhang, et al. Delivery of chemotherapeutic drugs in tumour cell-derived microparticles[J/OL].www.nature.com/naturecommunications, 2012-12-18/2019-10-09.
[24] Ma J, Zhang Y, Tang K, et al. Reversing drug resistance of soft tumor-repopulating cells by tumor cell-derived chemotherapeutic microparticles[J]. Cell Research, 2016, 26(6):713-727.
[25] Prevc A, Bedina Z A, Cemazar M, et al. Bystander effect induced by electroporation is possibly mediated by microvesicles and dependent on pulse amplitude, repetition frequency and cell type[J]. Journal of Membrane Biology, 2016, 249(5):1-9.
[26] Hill AF.Exosomes and microvesicles: methods and protocols, methods in molecular biology[M]. New York:Humana Press, 2017:1-33.
[27] Zhang Wei, Yu Zili, Wu Min, et al.Magnetic and folate functionalization enables rapid isolation and enhanced tumortargeting of cell-derived microvesicles[J].ACS Nano, 2017, 11:277-290.
[28] Chiriacò MS, Bianco M, Nigro A, et al.Lab-on-chip for exosomes and microvesicles detection and characterization[J]. Sensors (Basel), 2018, 18(10):3175.
[29] Santana SM, Antonyak MA, Cerione RA, et al.Microfluidic isolation of cancer-cell-derived microvesicles from hetergeneous extracellular shed vesicle populations[J].Biomed Microdevices, 2014, 16(6):869-877.
[30] Zhang Pan, Joo CY, Lim CT. Advances in Technologies for Purification and Enrichment of Extracellular Vesicles[J/OL].journals.sagepub.com/home/jla, 2019-05-14/2019-10-09.
[31] Chiang Chunyi, Chen C.Toward characterizing extracellular vesicles at a single-particle level[J]. Biomedical Science, 2019, 26(9):2-10.
[32] Vanderpol E, Hoekstra AG, Sturk A, et al.Optical and non-optical methods for detection and characterization of microparticles and exosomes[J]. Thrombosis and Haemostasis, 2010, 8:2596-2607.
[33] Ren Sen, Chen Jing, Duscher D, et al.Microvesicles from human adipose stem cells promote wound healing by optimizing cellular functions via AKT and ERK signaling pathways[J].Stem Cell Research & Therapy, 2019, 10(47):2-14.
[34] Sokolova V, Ludwigb A, Hornungb S, et al. Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy[J].Colloids and Surfaces B: Biointerfaces, 2011, 87(1):146-150.
[35] Rhian MT, Ernesto LS. Hypertension Methods and Protocols[M]. New York:Humana Press, 2017:326-336.
[36] Chandler W L. Measurement of microvesicle levels in human blood using flow cytometry[J]. Cytometry Part B: Clinical Cytometry, 2016, 90(4):326-336.
[37] RafaS, Monika BK, Jakub Z, et al.The methods of choice for extracellular vesicles (EVs) characterization[J].International Journal of Molecular Sciences, 2017, 18(1153):2-18.
[38] Lannigana J, Erdbrueggerb U.Imagingow cytometry for the characterization of extracellular vesicles[J].Methods, 2017, 112:55-67.
[39] van der Pol E, Coumans FA, Gardiner C, et al.Particle size distribution of exosomes and microvesicles determined by transmission electron micro -scopy, ow cytometry, nanoparticle tracking analysis, and resistive pulse sensing[J].Journal of Thrombosis and Haemostasis, 2014, 12:1182-1192.