The Preparation and Biological Study of Amphiphilic Glycopolypeptides as Liver-Targeted Theranostic Nanoparticles
Lai Shengsheng1#, Liu Qiancheng1, Jin Haoyu1, Liu Wenping1, Zhu Huier2*
1(School of Medical Instruments, Guangdong Food and Drug Vocational College, Guangzhou 510520, China) 2(Department of Emergency, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China)
Abstract：In this work, amphiphilic glycopolypeptides were synthesized by a sequential ring opening polymerization of ε-carbobenzyloxy-L-lysine N-carboxyanhydride and glutamic acid based N-carboxyanhydride monomer. Galactosyl sugar units as targeting ligands were conjugated to the polypeptides block via an efficient click reaction. The chemical structures of the obtained glycopolypeptides were characterized by Fourier transform infrared spectroscopy and nuclear magnetic resonance analysis. Glycopolypeptides base nanoparticles were prepared by dialysis method in aqueous solution, followed by characterized with fluorometry, transmission electron microscopy and dynamic light scattering. The biological activity was demonstrated by selective lectin binding experiments. The cytotoxicity of the glycopolypeptides was investigated by MTT test. Furthermore, liver targeted theranostic nanoparticles were prepared by using glycopolypeptides acted as nano-cargo to load near infrared dyes IR780. The cellular uptake, photothermal therapy effect and in vivo tumor imaging were investigated by using fluorescence imaging, quantitative flow cytometry and in vivo imaging system. Results indicated that the amphiphilic glycopolypeptides were obtained via the combination of ring open polymerization and “click” reaction. Own to their amphiphilic property, glycopolypeptides self-assembled into spherical nanoparticles with about 85 nm in diameter in an aqueous medium once the concentration was over 0.015 μg/mL. MTT results revealed that the glycopolypeptides nanoparticles was nontoxic to HepG2 cells and HUVECs even the concentration was up to 500 μg/mL. Fluorescence microscope revealed the specific recognition between glycopolypeptides and ricin agglutinin. According to the flow cytometry results, glycopolypeptides nanoparticles could deliver IR780 into the HepG2 cells effectively. Photothermal therapy study revealed that higher doses of IR-780 killed more tumor cells after laser irradiation at 808 nm. In vivo NIRF imaging shown that the theranostics nanoparticles were mainly accumulated in the tumor mass, even after 24 h, strong flourescence signals were still detected from the tumor mass. The glycopolypeptides were demonstrated promising tumor targeting theranostic nanocarriers.
赖胜圣, 刘虔铖, 金浩宇, 刘文平, 朱慧儿. 新型两亲性糖聚肽肝癌靶向诊疗一体化纳米粒子的制备及体内外实验研究[J]. 中国生物医学工程学报, 2019, 38(4): 455-463.
Lai Shengsheng, Liu Qiancheng, Jin Haoyu, Liu Wenping, Zhu Huier. The Preparation and Biological Study of Amphiphilic Glycopolypeptides as Liver-Targeted Theranostic Nanoparticles. Chinese Journal of Biomedical Engineering, 2019, 38(4): 455-463.
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