The Study on Fabrication and Property of 3D Printed Double-CrosslinkedScaffold MaterialsSilk Fibroin/Alginate/Mineralized Collagen for Bone Repair
Wang Di1& , Gan Fangjin1& , Lian Xiaojie1*, Huang Ruoxi1 , Zhang Siruo2 , Xu Rui1
1(Research Center for Nano-Biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China) 2(Shanxi Key Laboratory of Functional Proteins, Shanxi Jinbo Bio-Pharmaceutical Co., Ltd, Taiyuan 030032, China)
Abstract：Bone repair materials should have good osteogenic activity and mechanical properties to provide a good microenvironment for bone regeneration. The 3D printing technology can prepare scaffolds with designed porous structures, biological activity and mechanical properties. In this study, SF-GMA was synthesized by reacting silk fibroin (SF) with the glycidyl methacrylate (GMA) to introduce the photomymeric group, and then mixed with sodium alginate (SA) to construct a double crosslinking system. To improve the osteoblast activity and mechanical properties, the material was further blended with silk fibroin nanofibers (SFF) and mineralized collagen (HAC). Bone repair scaffolds were prepared by 3D printing technology. Experimental results showed that when the SF-GMA was in the content of 1~4 wt%, the mean value of compression elastic modulus was increased to 0.23~0.37 MPa for the composite scaffolds. After blended with HAC, the degradation ratio of the scaffolds decreased, and the mean degradation ratio was 22%~32% on the 28th day. In addition, after integrating SF-GMA and HAC, the proliferation ability of MC3T3-E1 increased with the increase of SF-GMA concentration. This study can provide certain theoretical and experimental basis for bone tissue repair.
王迪, 甘芳巾, 连小洁, 黄若希, 张斯若, 徐睿. 3D打印双交联丝素/海藻酸钠/矿化胶原骨修复支架材料的构建及性能研究[J]. 中国生物医学工程学报, 2025, 44(3): 325-334.
Wang Di, Gan Fangjin, Lian Xiaojie, Huang Ruoxi, Zhang Siruo, Xu Rui. The Study on Fabrication and Property of 3D Printed Double-CrosslinkedScaffold MaterialsSilk Fibroin/Alginate/Mineralized Collagen for Bone Repair. Chinese Journal of Biomedical Engineering, 2025, 44(3): 325-334.
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