Luo Zhiming1,2,3,4, Deng Guohao1, Wang Zhubing1, Zhang Yujie2,3,4, Zhang Yang1*, Wang Huanan2,3,4*
1(School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen 518055, Guangdong, China) 2(State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, Liaoning, China) 3(Frontiers Science Center for Smart Materials Oriented Chemical Engineering, Dalian University of Technology, Dalian 116024, Liaoning, China) 4(School of Bioengineering, Dalian University of Technology, Dalian 116024, Liaoning, China)
Abstract：Organ-on-a-chip is a biomimetic microphysiological system that recapitulates certain functions of tissues and organs by seeding live cells in delicately designed microfluidic chips. Organ-on-a-chip has a wide application in disease simulation, toxicity detection, new drug development and screening, personalized medical treatment, etc. The application of 3D printing organ-on-a-chip realizes the simplification and low cost of the chip manufacture and meet the delicate requirements of complex heterogeneous three-dimensional microenvironments. This review updated the recent progress in 3D printing organ-on-a-chip from the aspects of printing methods, printing inks and their biomedical applications. We reviewed the printing methods and ink materials for the construction of structural and functional units of organ chips and discussed the potential to develop more efficient one-step manufacture of organ chip. At last, we summarized the recent progress of using 3D-printed organ chip in bionic heart, liver, lung, kidney, nerve, tumor models, and outlook the future trend of organ-on-a-chip techniques.
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