Optimization of Cryoprotectants Addition and Removal Protocols Using Microfluidic Chip
Zhou Xinli1*, Du Yukun1, Yi Xingyue1, Dai Jianjun2, Zhang Defu2
1(Institute of Biothermal Technology, University of Shanghai for Science and Technology, Shanghai 200093, China) 2(Animal and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China)
Abstract：Cryoprotectants (CPA) addition and removal are essential steps in oocytes cryopreservation. In recent years, microfluidic chips have been used for addition and removal of CPA. The way of addition and removal in microfluidic chips will affect the survival rate and in vitro development of oocytes. In this paper, linear, convex and concave CPA loading or removal protocols were realized by adjusting injection models of microinjection pumps. Nine addition-removal combined protocols were achieved in the microfluidic system. The survival rate and in vitro development of oocytes with different CPA addition-removal protocols were investigated. Results showed that the survival rate (97.22%) and morula rate (46.03%) of porcine MII oocytes from concave loading-convex unloading protocol were significantly higher than that using other protocols(P < 0.05). There was a certain matching relationship between the addition and removal of CPA. This research provided a new solution for optimizing continuous addition-removal of CPA by microfluidic method.
周新丽, 杜羽琨, 衣星越, 戴建军, 张德福. 微流控法低温保护剂添加及去除线型优化研究[J]. 中国生物医学工程学报, 2019, 38(6): 719-725.
Zhou Xinli, Du Yukun, Yi Xingyue, Dai Jianjun, Zhang Defu. Optimization of Cryoprotectants Addition and Removal Protocols Using Microfluidic Chip. Chinese Journal of Biomedical Engineering, 2019, 38(6): 719-725.
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