Abstract：Transcranial ultrasound stimulation (TUS) and transcranial magneto-acoustic stimulation (TMAS) are effective in modulating motor cortex. Previous studies have mostly been conducted under anesthesia confined to the difficulty of tethering conscious animals, and the analysis of the attenuating modulatory effects of anesthesia has primarily focused on the central nervous system. In this study, spontaneous electromyography (EMG) and TUS/TMAS-induced EMG were recorded during isoflurane anesthesia in 24 mice, the effects of anaesthetize on spontaneous and induced EMG were analyzed quantitatively in terms of firing rate, latency, duration and amplitude. Experimental results showed that the frequency of spontaneous EMG decreased by 50% per cycle approximately and the duration decreased, as the concentration of isoflurane increased from 0.4% to 0.75%, indicating an inhibitory state. The induced-EMG success rates of TUS/TMAS both decreased by 50% and 70% respectively, with an average increase of 0.1 s in latency and a decrease of 0.3 s and 0.5 s in duration respectively, suggesting that the modulatory effect of TUS/TMAS on the motor cortex attenuated as the depth of anesthesia increased. According to the observed correlation between the firing rate and duration of spontaneous and induced EMG, it is rational to infer that the suppression of spontaneous EMG in mice under anesthesia was one of the factors contributing to the attenuated modulatory effect.
作者简介: #中国生物医学工程学会会员(Member, Chinese Society of Biomedical Engineering)
引用本文:
王茹茹, 周晓青, 赵渝卉, 刘煦, 刘志朋, 王欣, 殷涛. 异氟烷麻醉对小鼠自发肌电及TUS/TMAS诱发肌电的影响[J]. 中国生物医学工程学报, 2024, 43(1): 10-17.
Wang Ruru, Zhou Xiaoqing, Zhao Yuhui, Liu Xu, Liu Zhipeng, Wang Xin, Yin Tao. Effects of Isoflurane Anesthesia on Spontaneous and TUS/TMAS Induced Electromyography in Mice. Chinese Journal of Biomedical Engineering, 2024, 43(1): 10-17.
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