Review of the Portable Fully Integrated Nucleic Acid Analysis System
Li Nan1#, Xu Youchun1,2*, Cheng Jing1,2#*
1(State Key Laboratory of Membrane Biology, Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China) 2(National Engineering Research Center for Beijing Biochip Technology, Beijing 102206, China)
Abstract：Nucleic acid testing plays important roles in many fields, such as clinical diagnosis, public health, food safety, molecular breeding, and forensic identification. In standard nucleic acid testing laboratories, physically separated spaces are required for sample preparation, nucleic acid extraction, and amplification, and many manual operations and supporting equipment are involved, which makes the entire detection process tedious, inefficient, and prone to cross-contamination. Therefore, the trend of nucleic acid detection and analysis is to develop fully integrated and automated nucleic acid detection systems. With the development of microfluidic technology, it is now possible to construct a fully integrated portable nucleic acid analysis system to satisfy the needs of point-of-care testing and/or on-site detection. This article reviewed the technical characteristics of the fully integrated portable nucleic acid analysis system from the aspects of biotechnology and microfabrication technology, compared some fully integrated representative systems for nucleic acid analysis, and proposed its technical characteristics of multi-disciplinarity, diversification of technology paths, full integration and functional expansion. At the end of the article, we concluded the challenges of complex molecular diagnostic requirement, high requirement for rapid detection, fully automatic operation and low cost for the portable fully integrated nucleic acid detection system technology, as well as discussed the possible future development.
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