羊膜基质/聚丙烯复合补片的制备及其生物相容性评价

doi:10.3969/j.issn.0258-8021.2020.03.17

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摘要制备脱细胞羊膜基质, 运用物理方法将其复合于临床用聚丙烯补片, 以形成具有更好组织相容性的生物补片。通过细胞培养, 以聚丙烯膜为对照, 评价复合生物补片的细胞相容性;通过动物皮下植入实验, 以评价复合补片的组织相容性。结果表明, 复合补片羊膜基质表面上的成纤维细胞生长和形态均好于聚丙烯表面, 细胞增殖较快, 增殖率超过聚丙烯表面的50%。动物实验显示, 复合补片周围增生少, 无明显纤维包膜形成, 初期在植入周围有淋巴细胞等炎性细胞浸润, 随着羊膜逐渐降解, 4周时可见毛细血管新生;而对于聚丙烯材料, 植入处纤维包囊明显, 炎性情况也较严重。经羊膜基质复合的聚丙烯补片具有良好的细胞和组织相容性。

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	葛芬芬
	林静
	胡慧洁
	吴徐金
	沈鑫炜
	周密
	竺亚斌

关键词 ：羊膜基质, 聚丙烯补片, 细胞相容性, 组织相容性, 生物补片

Key words： polypropylene mesh human amnion membrane decellularization biocompatibility surgery mesh

收稿日期: 2018-09-17

PACS:

R318

基金资助:宁波市自然科学基金(2017A610165, 2017A610259)

通讯作者: *, E-mail: gefenfen1982@126.com;zhuyabin@nbu.edu.cn

引用本文:

葛芬芬, 林静, 胡慧洁, 吴徐金, 沈鑫炜, 周密, 竺亚斌. 羊膜基质/聚丙烯复合补片的制备及其生物相容性评价[J]. 中国生物医学工程学报, 2020, 39(3): 380-384.
Ge Fenfen, Lin Jing, Hu Huijie, Wu Xujin, Shen Xinwei, Zhou Mi, Zhu Yabin. The Fabrication and Biocompatibility of Human Amnion Membrane Matrix/Polypropylene Composite Mesh. Chinese Journal of Biomedical Engineering, 2020, 39(3): 380-384.

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http://cjbme.csbme.org/CN/10.3969/j.issn.0258-8021.2020.03.17 或 http://cjbme.csbme.org/CN/Y2020/V39/I3/380

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