The Cytocompatibility of Ctenopharyngodon Idellus Fishskin Collagen with NIH3T3 Fibroblasts
Yu Lan1, Wang Haibo2, Chen Zhuo1, Fang Cheng1*
1(Laboratory of Transplant Engineering,Wuhan Polytechnical University,Wuhan 430023,China) 2(College of Chemical and Environmental Engineering,Wuhan Polytechnical University,Wuhan 430023,China)
Abstract：This study is aimed to test the cytocompatibility of fishskin acid-soluble collagen (FASC) of ctenopharyngodon idellus in vitro. The proliferation rates of NIH3T3 fibroblasts were detected using different concentrations of FASC solutions or coating gums by MTT experiment (n=6). The chemotaxis of fibroblasts towards FASC solutions were tested by chemotactic experiments of transwell (n=3). The cellular permeabilities in different concentrations of FASC coating gums were detected by invasive experiments of transwell (n=3). FASC solutions promote proliferation of NIH3T3 fibroblasts and the growth activity of the fibroblasts was dose-dependent on FASC. The proliferation rates of 16 μg/mL FASC solution in 48 and 96 h were (63.7±7.9)% and (87.3±8.7)%, that showed significant differences compared with the control group (P<0.001). NIH3T3 fibroblasts grew on FASC coating gums without significant differences compared with the control group (P>0.05). The chemotatic indexes showed significant differences between the concentration gradient groups of FASC solutions and the control group(P<0.05), which indicated that FASC has chemotaxis to fibroblasts. Invasion experiments showed fibroblasts could pass through FASC coating gums. These experiments confirmed that FASC possesses favourable cytocompatibility with NIH3T3 fibroblasts.
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