Experimental Mechanism Study on Killing of Chemoresistant Tumor Cells Exposed to High Voltage Nanosecond Pulse Field
Liu Hongmei1, Wang Li2, Dong Shoulong1, Ma Jianhao1, Wang Yilin1, Yao Chenguo1#*
1(State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University, Chongqing 400044, China); 2(Institute of Immunology PLA & Department of Immunology, Army Medical University, Chongqing 400038, China)
Abstract：Tumor seriously endanger the lives and health of human people. Chemoresistance is one of the main barriers to successful tumor treatment. In order to explore whether high voltage nanosecond pulsed electric fields can be equally sensitive to chemotherapy-resistant tumor cells compared with its homology tumor cells, lung cancer cell line A549 and cisplatin chemotherapy-resistant A549/R were used as the research object in this paper. After exposing to the 80 nanosecond pulses with a pulse width of 200 ns and a field strength of 5-15 kV/cm, the activity of cell, apoptosis and necrosis and ablation effect were analyzed and compared in groups. The results showed that the survival rate of A549/R was 5.65%, 7.78% and 2.80% respectively when exposed topulses electric field with strength of 8kV/cm, 10 kV/cm and 15 kV/cm, which was significantly lower than that of A549 cells (48.31%, 26.8%, and 5.96%, respectively for 8 kV/cm, 10 and 15 kV/cm). The induced apoptosis and necrosis rates were also significantly higher than A549 (P<0.05); In addition, when exposed to 80 pulses(200 ns, 15 kV/cm), the ablation area of single-layer cells for A549/R was 1.59 times that of A549 cells, and the ablation threshold for A549/R (8 kV/cm) is significantly lower than that of A549 (13 kV/cm). Therefore, the nanosecond pulsed electric field with a pulse width of 200 ns could preferentially kill A549/R, inducing a higher rate of apoptosis, ablation area and a lower ablation threshold. The experimental results showed that high-voltage nanosecond pulse could preferentially act on chemotherapy-resistant tumor cells.
作者简介: #中国生物医学工程学会会员(Member, Chinese Society of Biomedical Engineering)
引用本文:
刘红梅, 王莉, 董守龙, 马剑豪, 王艺麟, 姚陈果. 高压纳秒脉冲电场杀伤化疗抗性肿瘤细胞的实验机理研究[J]. 中国生物医学工程学报, 2019, 38(3): 332-338.
Liu Hongmei, Wang Li, Dong Shoulong, Ma Jianhao, Wang Yilin, Yao Chenguo. Experimental Mechanism Study on Killing of Chemoresistant Tumor Cells Exposed to High Voltage Nanosecond Pulse Field. Chinese Journal of Biomedical Engineering, 2019, 38(3): 332-338.
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