Study on the Antibacterial Activity and Biocompatibility of TiN Coated Ti-6Al-4V Implanted with Cu2+
Chu Shanshan1, Wan Rongxin2, Lv Xiaofei1, Gu Hanqing2*, Peng Cheng1
1.(The Second Hospital of Tianjin Medical University, Tianjin 300211, China) 2.(Tianjin Institute of Urological Surgery, Tianjin Medical University, Tianjin 300211, China)
Abstract：The aim of this work is to investigate and compare the antibacterial activity and biocompatibility of Ti-6Al-4V carrying TiN implanted with Cu2+ and Ag+ respectively. L929 cells were incubated on blank control group - Ti-6Al-4V (titanium alloy group), negative control group -TiN coated Ti-6Al-4V (TiN group), positive control group -TiN coated Ti-6Al-4V surface conjugated with Ag (Ag group), experimental group - TiN coated Ti-6Al-4V surface conjugated with Cu (Cu group). The cytoskeleton morphology of the cells was observed by laser confocal scanning. Scanning electron microscope was used to observe the spreading, adhesion and growth of cells on the substrates. Cell proliferation was measured by CCK-8 assay. The straphylococcus aureus solution was dropwise added to the surface of each group of samples. After 24 h incubation, the number of viable bacteria colonies on the surface of the material was determined by the plate count method. The contact angle reflects the hydrophilzicity of the material. XPS reflects the surface elements composition of the material and ICP-MS can reflect the amount of ion precipitation of the target element of the material. CLSM and SEM: The cells in the Ag group and the Cu group were dense, meanwhile spread and adhered on the substrates, showing slab pseudopods and filopodia. CCK-8 result: After 1 day, 3 day, and 5 day incubation, viable cells in Ag group and Cu group were more than those on the titanium alloy group and the TiN group, which proved that the two groups had no apparent toxicity. Plate count method: The Ag group and Cu group had fewer colonies; the bacteriostasis rate was 91%±2% and 87%±2% respectively. CLSM and SEM: The bacteria adhered on Cu group and Ag group was less than those on titanium alloy group and TiN group. The integrity of bacterial wall was damaged and even broken. The Cu group and Ag group showed similar cell compatibility and antibacterial property.
褚珊珊, 万荣欣, 吕晓飞, 顾汉卿, 彭诚. TiN涂覆的Ti-6Al-4V注入Cu2+后的抑菌性与细胞相容性研究[J]. 中国生物医学工程学报, 2019, 38(2): 208-215.
Chu Shanshan, Wan Rongxin, Lv Xiaofei, Gu Hanqing, Peng Cheng. Study on the Antibacterial Activity and Biocompatibility of TiN Coated Ti-6Al-4V Implanted with Cu2+. Chinese Journal of Biomedical Engineering, 2019, 38(2): 208-215.
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