Study on the Preparation and Blood Compatibility of Acellular Vascular Scaffold with Triton-x100 and Salvianolic Acid B
Zhao Liang1,2, Li Xiafei3, Li Chengcheng1, Yan Huanhuan1, Zhang Qiqing1*
1.(Life science and Health Research Institute, College of life Sciences and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, China) 2.(PKU-HKUST Shenzhen-Hongkong Institution, Shenzhen 518057,Guangdong, China); 3.(School of Biomedical Engineering, Xinxiang Medical University, Xinxiang 453003, Henan, China)
Abstract：To compare the physicochemical properties and blood compatibility of two kinds of acellular vascular stents prepared by traditional Triton-x100 method and salvianolic acid B with Triton-x100. To explore the prospect of the application of salvianolic acid B post-treated acellular vascular stents in the treatment of cardiovascular and cerebrovascular diseases and the development of tissue engineering vascular materials. A total of 16 SD rats, male and 7 weeks old, with a mass of 250 g, were included and randomly divided into 2 groups, 8 in each group. Two kinds of acellular vascular scaffolds prepared by Triton-x100 method (Tx group) and Triton-x100 adding salvianolic acid B method (Tx-sal group) were implanted. The macroscopical morphology, hydrophilicity, in vitro hemolysis rate and the determination of coagulation time of complex calcification were investigated. The blood compatibility of two groups of acellular scaffold was compared on the basis of a series of experiments. The contact angle of Tx group was (76.36±4.647), Tx-sal group was (71.26±3.553), and Tx group was slightly higher than Tx-sal group, which didn′t have significant difference in statistics. Hemolysis rate in the group of Tx-sal was 1.5% and hemolysis rate in the group of Tx was 2.1%. The coagulation time of recalcification in Tx group was (212±11.32) s, and that of Tx-sal group was (231±13.53) s. There was significant difference in the coagulation time between Tx group and Tx-sal group. Group Tx-sal had delayed coagulation time and had better anticoagulation when compared with group Tx. The platelet count in the group of Tx-sal was lower than that in the group of Tx. The level of complement activation was lower in the group of Tx-sal. In conclusion, the acellular scaffold with salvianolic acid B had better blood compatibility than the acellular scaffold prepared by traditional Triton-x100 method.
赵亮, 李霞飞, 李成成, 闫欢欢, 张其清. 应用Triton-x100加丹参酚酸B制备脱细胞血管支架及其血液相容性研究[J]. 中国生物医学工程学报, 2019, 38(2): 201-207.
Zhao Liang, Li Xiafei, Li Chengcheng, Yan Huanhuan, Zhang Qiqing. Study on the Preparation and Blood Compatibility of Acellular Vascular Scaffold with Triton-x100 and Salvianolic Acid B. Chinese Journal of Biomedical Engineering, 2019, 38(2): 201-207.
