Abstract:This paper introduced a portable potentiostat platform based on AVR microcontroller, which was used for electrochemical detection of trace vitamin B2. The platform adopted the high-precise conversion chip AD7705 and DAC8831 and used a microcontroller ATmega16 as a controlling core. The integrated operational amplifier was designed by OPA211 as the core device. The software design adopted optimized calibration logic. Therefore, the potentiostat output was effectively controlled, and the functions of setting the potentiostatic and measuring the polarization current parameter were implemented. Testing results showed that the potentiostat potential error was controlled within the millivolt, the maximum error was about 1%, and the test current limit value was less than 1 μA. The stability and sensitivity of the portable potentiostat platform was also analyzed compared with the CHI660C electrochemical instrument. The linearity between the peak current and the concentration of vitamin B2 was measured by the platform experiment, and the correlation coefficient was 0.994. The rapid detection of vitamin B2 in food was preliminarily verified.
徐 莹, 罗玉林, 郭 淼. 检测微量维生素B2的便携式恒电位仪平台设计[J]. 中国生物医学工程学报, 2017, 36(2): 187-194.
Xu Ying, Luo Yulin, Guo Miao. Design of the Portable Potentiostat for the Detection of Trace Level of Vitamin B2. Chinese Journal of Biomedical Engineering, 2017, 36(2): 187-194.
[1] Bai Jin, Ndamanisha JC, Liu Lin, et al. Voltammetric detection of riboflavin based on ordered mesoporous carbon modified electrode [J]. Journal of Solid State Electrochemistry, 2010, 14 (12):2251-2256. [2] Wongkaew N, Kirschbaum SEK, Surareungchai W, et al. A Novel Three-Electrode System Fabricated on Polymethyl Methacrylate for On-Chip Electrochemical Detection [J]. Electroanalysis, 2012, 24 (10):1903-1908. [3] Duwe M, Chen T. Low power integrated potentiostat design for μ electrodes with improved accuracy [C]//IEEE 54th International Midwest Symposium on Circuits and Systems (MWSCAS). Seoul: IEEE, 2011:1-4. [4] 钟海军,邓少平. 恒电位仪研究现状及基于恒电位仪的电化学检测系统的应用[J]. 分析仪器, 2009(02): 1-5. [5] 潘银松,张成伟,张仁富,等. 基于微生物传感器的恒电位仪电流积分电路的设计[J]. 传感技术学报,2009,22(3):307-310. [6] 姚毓升,解永平,文涛. 三电极电化学传感器的恒电位仪设计[J]. 仪表技术与传感器, 2009( 9):23-25. [7] Ayers S, Gillis KD, Lindau M, et al. Design of a CMOS Potentiostat Circuit for Electrochemical Detector Arrays [J]. IEEE Transactions on Circuits and Systems, 2007, 54 (4):736-744. [8] Huang Chunyueh. Design and implementation of a voltammetry potentiostat with wide dynamic current range measurement for electrochemical biosensors [J]. Analog Integrated Circuits and Signal Processing, 2014,81 (1):205-214. [9] Jin Yang, Wang Hong, Lv Zhengliang, et al. Reliable Remote-Monitoring Electrochemical Potentiostat for Glucose Measurements [J]. Tsinghua Science & Technology, 2009, 14 (5):593-600. [10] Kim JH, Lee JY, Jin JH, et al. A fully microfabricated carbon nanotube three-electrode system on glass substrate for miniaturized electroche micalbiosensors [J]. Biomedical Microdevices, 2012, 14 (3):613-624. [11] Cardoso JL, Menezes LED, Emeri JLD, et al. Construction and Evaluation of an Opto-Coupled Potentiostat with a PC/PDA Interface [J]. Instrumentation Science & Technology, 2008, 36 (6):623-635. [12] 王兆雨,吴效明,刘仲明. 基于C8051F020的三电极电化学检测系统设计[J]. 中国医学物理学杂志,2013,30(1):3909-3912. [13] Levine PM, Gong P, Levicky R, et al. Active CMOS Sensor Array for Electrochemical Biomolecular Detection [J]. IEEE Journal of Solid-State Circuits, 2008, 43(8):1859-1871. [14] Ramfos I, Vassiliadis N, Blionas S, et al. A compact hybrid-multiplexed potentiostat for real-time electrochemical biosensing applications [J]. Biosensors & bioelectronics, 2013, 47(18):482-489. [15] 王泽华,曾冬冬,张欢,等. 电沉积纳米金修饰的16通道电流型PSA免疫传感器的制备[J]. 中国生物医学工程学报, 2015, 34(1):55-61. [16] 胡金夫,徐铭恩,徐莹,等. 基于细胞三维受控组装技术的细胞芯片构建[J]. 中国生物医学工程学报,2012,31(3):374-381.