Abstract：Th differentiation play a key role in modulating immune response, which is mainly controlled by dendritic cells (DCs). Carbon nanotubes are unique nanomaterials composed of carbon element with hollow tube structures, which could be used as antigen carrier. The interaction of carbon nanotubes with DCs is key factor modulating following immune responses. This work studied the interaction between oxidized multiwalled carbon nanotubes (CNT) and DCs, and the mixture of CNT and allergic antigen peptide OVA323-339 (O-3) on DCs and DCs-mediated Th1/Th2 responses. Our results showed that CNT was engulfed by DCs, and the expression of MHCII, CD86 and TNF-α of DCs was unchanged by CNT. The mixture of CNT/O-3 treated DCs induced increased CD8+ T cell proliferation. The proliferation ratio of CD8+ T cells increased from 28.7% of control to 40.6%, 41.3% and 29.6%. Th2 cytokines expression of IL-4, IL-10 and IL-13 was significantly inhibited. The proliferation ratio of CD4+ T cells were decreased from 54.5% of control to 38.9%, 46.6%, and 39.8%. All the results indicated that CNT did not influence the maturation of DCs but promote Th1 polarization of T cells by inhibiting the Th2 cytokines expression of allergic peptide antigen.
代亚丽,聂欣,刘健,孟洁,许海燕. 碳纳米管对树突状细胞成熟和T细胞分化作用研究[J]. 中国生物医学工程学报, 2017, 36(4): 464-469.
Dai Yali, Nie Xin, Liu Jian, Meng Jie, Xu Haiyan. Carbon Nanotubes Influence Dendritic Cells Maturation and T Cell Polarization. Chinese Journal of Biomedical Engineering, 2017, 36(4): 464-469.
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