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| Effects of Nano-Hydroxyapatite/Chitosan (N-HA/CS) on MC3T3-E1 Cell and Metabolic Organ in SD Rats |
1 (Key Laboratory for Biomechanics and Mechanobiology of the Ministry of Education, School of Biological Sciences and Medical Engineering, Beihang University, Beijing 100191, China
2 National Research Center for Rehabilitation Technical Aids, Beijing 100176, China |
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Abstract Composite conmposed of nanohydroxyapatite and chitosan (n-HA/CS) is widely used in the field of bone repair and alternative. To investigate the degradation effects of nHA/CS on metabolism organs in SD rat and analyze the possible mechanism, particulate n-HA/CS was prepared by precipitation and characterized by IR and XRD. Serum biochemical parameters measurement and histopathological analysis were performed to evaluate the composite toxicity after 8 weeks of composite implantation (100 mg/kg). Results show that blood urea nitrogen (BUN) levels was 29.6 mmol/L, creatinine (CR) levels was 66.9 umol/L, total bilirubin (T-B) and indirect bilirubin (I-B) levels were also increased to 1.79 umol/L and 1.32umol/L. Compared with that of control, there were significant differences (P≤0.05). A large number of apoptotic instead of necrosis cells were observed in the liver and renal proximal tubule tissue. It is concluded that the toxicity of n-HA/CS is attributable to the cells apoptosis.
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［1］Ou KL, Wu J, Lai WF, et al. Effects of the nanostructure and nanoporosity on bioactive nanohydroxyapatite / reconstituted collagen by electrodeposition ［J］. J Biomed Mater Res A, 2010, 92 (3):906-912.
［2］Albrecht C, Scherbart AM, Berlo DV, et al. Evaluation of cytotoxic effects and oxidative stress with hydroxyapatite dispersions of different physicochemical properties in rat NR8383 cells and primary macrophages ［J］. Toxicol In Vitro, 2009, 23 (3):520-530.
［3］Liao S, Tamura K, Zhu Y, et al. Human neutrophils reaction to the biodegraded nanohydroxyapatite/collagen and nanohydroxyapatite / collagen /poly (Llactic acid) composites ［J］. J Biomed Mater Res A, 2006, 76(4):820-825.
［4］Kashiwazaki H, Kishiya Y, Matsuda A, et al. Fabrication of porous chitosan/hydroxyapatite nanocomposites: Their mechanical and biological properties ［J］. Biomed Mater Eng, 2009, 19(2):133-140.
［5］Zhou Gang, Li Yubao, Zhang Li et al., Preparation and characterization of nanohydroxyapatite/ chitosan / konjac glucomannan composite ［J］. Journal of Biomedical Material Research Part A, 2007, 83: 931-939.
［6］Watari F, Abe S, Koyama C, et al. Behavior of in vitro, in vivo and internal motion of micro/nano particles of titanium, titanium oxides and others ［J］. Journal of the Ceramic Society of Japan, 2008, 116(1349):1-5.
［7］Motskin M, Wright DM, Muller K, et al. Hydroxyapatite nano and microparticles: correlation of particle properties with cytotoxicity and biostability ［J］. Biomaterials, 2009, 30(19):3307-3317.
［8］Lu Xiaoyan, Jin Tingting, Jin Yachao, et al. Toxicogenomic analysis of the particle dose and sizeresponse relationship of silica particlesinduced toxicity in mice ［J］. Nanotechnology, 2013, 24(1): 015106.
［9］Xu Pengjuan, Xu Jing, Liu Shichang, et al. Nano copper induced apoptosis in podocytes via increasing oxidative stress ［J］. J Hazard Mater, 2012, 241-242:279-286.
［10］Yeo MK, Pak SW. Exposing zebrafish to silver nanoparticles during caudal fin regeneration disrupts caudal fin growth and p53 signaling ［J］. Molecular＆Cellular Toxicology, 2008, 4(4): 311-317.
［11］Chen Jinyuan, Dong Xia, Zhao Jing, et al. In vivo acute toxicity of titanium dioxide nanoparticles to mice after intraperitioneal injection ［J］. J Appl Toxicol, 2009, 29(4):330-337.
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