Effects of Nano-Hydroxyapatite/Chitosan (N-HA/CS) on MC3T3-E1 Cell and Metabolic Organ in SD Rats
1 (Key Laboratory for Biomechanics and Mechanobiology of the Ministry of Education, School of Biological Sciences and Medical Engineering, Beihang University, Beijing 100191, China
2 National Research Center for Rehabilitation Technical Aids, Beijing 100176, China
Abstract Composite conmposed of nanohydroxyapatite and chitosan (n-HA/CS) is widely used in the field of bone repair and alternative. To investigate the degradation effects of nHA/CS on metabolism organs in SD rat and analyze the possible mechanism, particulate n-HA/CS was prepared by precipitation and characterized by IR and XRD. Serum biochemical parameters measurement and histopathological analysis were performed to evaluate the composite toxicity after 8 weeks of composite implantation (100 mg/kg). Results show that blood urea nitrogen (BUN) levels was 29.6 mmol/L, creatinine (CR) levels was 66.9 umol/L, total bilirubin (T-B) and indirect bilirubin (I-B) levels were also increased to 1.79 umol/L and 1.32umol/L. Compared with that of control, there were significant differences (P≤0.05). A large number of apoptotic instead of necrosis cells were observed in the liver and renal proximal tubule tissue. It is concluded that the toxicity of n-HA/CS is attributable to the cells apoptosis.
WANG Li Ting1,2 ZHOU Gang1 FAN Yu Bo1*. Effects of Nano-Hydroxyapatite/Chitosan (N-HA/CS) on MC3T3-E1 Cell and Metabolic Organ in SD Rats[J]. journal1, 2013, 32(5): 595-600.
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