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Detecting Neuronal Unit Spikes during Electrical Stimulation Periods |
College of Biomedical Engineering and Instrumentation Science, Key Laboratory of Biomedical Engineering of Ministry of Education, Zhejiang University, Hangzhou 310027, China |
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Abstract Deep brain stimulation (DBS) has been used to treat disorders in clinical practice, such as Parkinson’s disease of central nervous system. However, the mechanisms of DBS are not clear yet. In order to investigate the changes of action potential (i.e., unit spike) firings of individual neurons during the periods of electrical stimulation, we designed an interpolation method to remove stimulation artifacts together with evoked potentials with large amplitudes. Thresholds of signal slope were used to determine the end points of interpolation segment thereby minimizing the nonusable period (NP) of spike detection. The method was used to analyze the spike activity during either orthodromic or antidromic stimulation of hippocampal CA1 region in rat brain. Results showed that the NP immediately following stimulation could be shortened by ~8 ms using the method. A further decrease of the influence was achieved by using spline interpolation instead of linear interpolation. The novel method can be used to detect spike signals during the periods of high frequency stimulation efficiently, providing a simple and practicable method to investigate the neuronal activity during DBS.
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