电刺激期间神经细胞单元锋电位的检测

doi:10》3969/j.issn.0258-8021. 2013 0404

Abstract
Figure/Table
References (0)
Related Citation (2)

Download: PDF (1204 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Deep brain stimulation (DBS) has been used to treat disorders in clinical practice, such as Parkinson’s disease of central nervous system. However, the mechanisms of DBS are not clear yet. In order to investigate the changes of action potential (i.e., unit spike) firings of individual neurons during the periods of electrical stimulation, we designed an interpolation method to remove stimulation artifacts together with evoked potentials with large amplitudes. Thresholds of signal slope were used to determine the end points of interpolation segment thereby minimizing the nonusable period (NP) of spike detection. The method was used to analyze the spike activity during either orthodromic or antidromic stimulation of hippocampal CA1 region in rat brain. Results showed that the NP immediately following stimulation could be shortened by ～8 ms using the method. A further decrease of the influence was achieved by using spline interpolation instead of linear interpolation. The novel method can be used to detect spike signals during the periods of high frequency stimulation efficiently, providing a simple and practicable method to investigate the neuronal activity during DBS.

Key words： deep brain stimulation artifact population spike unit spike

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	FENG Zhou Yan^* XIAO Qian Jiang HU Zhen Hua

Cite this article:

FENG Zhou Yan^* XIAO Qian Jiang HU Zhen Hua. Detecting Neuronal Unit Spikes during Electrical Stimulation Periods[J]. journal1, 2013, 32(4): 403-410.

URL:

http://cjbme.csbme.org/EN/10》3969/j.issn.0258-8021. 2013 0404 OR http://cjbme.csbme.org/EN/Y2013/V32/I4/403

［1］Montgomery EB Jr, Gale JT. Mechanisms of action of deep brain stimulation (DBS)［J］. Neurosci Biobehav Rev, 2008, 32(3): 388-407.
［2］Kuhn J, Mller M, Müller U, et al. Deep brain stimulation for the treatment of addiction［J］. Addiction, 2011, 106(8): 1536-1537.
［3］Datta A, Baker JM, Bikson M, et al. Individualized model predicts brain current flow during transcranial directcurrent stimulation treatment in responsive stroke patient［J］. Brain Stimul, 2011, 4(3): 169-174.
［4］Buzsaki G. Largescale recording of neuronal ensembles［J］. Nat Neurosci, 2004, 7(5): 446-451.
［5］Erez Y, Tischler H, Moran A, et al. Generalized framework for stimulus artifact removal［J］. J Neurosci Methods, 2010, 191(1): 45-59. 
［6］Wagenaar DA, Potter SM. Realtime multichannel stimulus artifact suppression by local curve fitting［J］, J Neurosci Methods, 2002, 120(2): 113-120.
［7］Nam Y, Brown EA, Ross JD, et al. A retrofitted neural recording system with a novel stimulation IC to monitor early neural responses from a stimulating electrode［J］. J Neurosci Methods, 2009, 178(1): 99-102.
［8］Kloosterman F, Peloquin P, Leung LS. Apical and basal orthodromic population spikes in hippocampal CA1 in vivo show different origins and patterns of propagation［J］. J Neurophysiol, 2001, 86 (5): 2435-2444.
［9］封洲燕, 光磊, 郑晓静, 等. 应用线性硅电极阵列检测海马场电位和单细胞动作电位［J］. 生物化学与生物物理进展, 2007, 34(4): 401-407.
［10］Takekawa T, Isomura Y, Fukai T. Accurate spike sorting for multiunit recordings［J］. Eur J Neurosci, 2010, 31(2): 263-272.
［11］Kaneko H, Suzuki SS, Okada J, et al. Multineuronal spike classification based on multisite electrode recording, wholewaveform analysis, and hierarchical clustering［J］. IEEE Trans Biomed Eng, 1999, 46(3): 280-290.
［12］Heffer LF, Fallon JB. A novel stimulus artifact removal technique for highrate electrical stimulation［J］. J Neurosci Methods, 2008, 170(2): 277-284. 
［13］Winchman, T. A digital averaging method for the removal of stimulus artifacts in neurophysiologic experiments［J］. J Neurosci Methods, 2000, 98(1): 57–62.
［14］Hashimoto T, Elder CM, Vitek JL. A template subtraction method for stimulus artifact removal in highfrequency deep brain stimulation［J］. J Neurosci Methods, 2002, 113(2): 181-186. 
［15］封洲燕, 王静, 汪洋, 等. 神经元锋电位信号滤波频率的选择［J］. 浙江大学学报工学版, 2012, 46 (2): 351-358. 
［16］Dunn S, Constantinides A, Moghe PV, 封洲燕译. 数值方法在生物医学工程中的应用［M］. 北京: 机械工业出版社, 2009: 317-324.
［17］Brown EA, Ross JD, Blum RA, et al. StimulusArtifact Elimination in a MultiElectrode System［J］. IEEE Trans Biomed Circuits Syst, 2008, 2(1): 10-21.
［18］Montgomery EB, Gale J, Huang H. Methods for isolating extracellular action potentials and removing stimulus artifacts from microelectrode recordings of neurons requiring minimal operator intervention［J］. J Neurosci Methods, 2005, 144(1): 107-125. 
［19］Wichmann T, Devergnas A. A novel device to suppress electrical stimulus artifacts in electrophysiological experiments［J］. J Neurosci Methods, 2011, 201(1): 1-8.