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Cell Membrane Damage by Ultrasound in Combination with Protoporphyrin Ⅸ on S180 Tumor Cells |
College of Life Sciences, Shaanxi Normal University. Xian 710062, China |
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Abstract The effects of 1 μg/mL protoporphyrin Ⅸ(PpⅨ)combined with focused ultrasound at the frequency of 1.1 MHz and intensity of 3 W/cm2 on the damage of cell membrane of S180 tumor cell were studied in this paper. Results showed that the ultrasound combined with PpⅨ induced more serious damage of the cells membrane than the ultrasound or the PpⅨ alone. We demonstrated that acid phosphatase activity of the treatment group of ultrasound combined with PpⅨ was decreased from 0.63 ± 0.03 to 0.34 ± 0.01 compared with that of the control group. The structures of cell membrane were destroyed seriously with disappearance of microvilli and partial loss of cytoplasmic constituents in the observation of scanning electron microscopy. Furthermore, after the treatment, the activity of Na+-K+-ATPase, Ca2+-ATPase, the content of membrane sialic acid (SA) and intracellular GSH were all reduced significantly (P<0.05). The data indicated that part of membrane proteins was lost. It was also shown that the level of mitochondrial membrane potential decreased significantly, the mean fluorescence intensity of Rhodamin 123 was decreased from 14 082.78± 840.40 to 7 801.53± 409.15. And cytochrome C oxidase activity was reduced to 0.008 U/min/mg. The results indicated that the mitochondria maybe the target of ultrasound PpⅨ on S180 tumor cells.
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