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Effect of Cryoprotectants and Cooling Rates on Young’s Modulus of Porcine Articular Cartilage
Institute of Biothermal Science, University of Shanghai for Science & Technology, Shanghai 200093, China
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Abstract  To explore the effects of cryoprotectants, concentrations and cooling rates on Young’s modulus of porcine articular cartilage (AC), control-force mode of dynamic mechanical analysis (DMA) was used. Cooling rates were set as 1, 10 and 20℃/min. Dimethyl sulphoxide (DMSO), glycerine and 1,2-dihydroxypropane were chosen to be CPAs and the effects of their concentrations [i.e. 10%, 20% , 30%, 40%, 50%, 60% and 80%(V/V)] on Young's modulus of porcine articular cartilage were studied. The results showed that: 1) The Young's modulus was increased with the increase of CPA concentration within the low and medium concentration range [less than 40%(V/V)]. While within high concentration range [more than 40%(V/V)] the Young's modulus was decreased with the increasing of CPA concentration. For example, when the cooling rate was 1℃/min, the Young's modulus of sample treated with different DMSO concentrations were 1.412, 2.031, 2.331, 1.991, 2.227 and 1.868 MPa for 10%, 30%, 40%, 50%, 60% and 80%(V/V), respectively. 2) Within the low and medium CPA concentration range, slow cooling rate was beneficial to preserve Young's modulus of AC. For example, when the cooling rate was 1℃/min, the Young's modulus of sample treated with 40%(V/V) DMSO was 2.331 MPa, and 2.151 MPa for 10℃/min. 3) At the concentration range of 30%~60%(V/V), DMSO was the best one to protect the Young’s modulus of AC, glycerin was the second best one, and 1, 2-dihydroxypropan exhibited the lowest performance.
Key wordsarticular cartilage      dynamic mechanical analyzer      Young's modulus      cryoprotectants      cooling rate     
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SUN Hui Jun XU Yi* LV Ya
Cite this article:   
SUN Hui Jun XU Yi* LV Ya. Effect of Cryoprotectants and Cooling Rates on Young’s Modulus of Porcine Articular Cartilage[J]. journal1, 2013, 32(1): 53-60.
URL:  
http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2013.01.008     OR     http://cjbme.csbme.org/EN/Y2013/V32/I1/53
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