表面矿化修饰煅烧骨/BMP<sub>2</sub>活性多肽复合MC3T3-E1细胞构建组织工程骨

doi:10.3969/j.issn.0258-8021. 2014. 03.012

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Abstract The aim of this study is to investigate the osteogenesis of tissue engineered bone constructed by sintered bone with surface mineralization modification/BMP2related peptide (P24) composite with MC3T3-E1 cells in vivo. The biomaterials were divided into three groups: Group A (sintered bone with surface mineralization modification / P24 composite); Group B (sintered bone with surface mineralization modification) and Group C (sintered bone). The MC3T3-E1 cells were induced by osteogenic medium and then seeded into the three materials. The cellmaterial samples were observed by inverted microscopy and environment scanning electron microscopy (ESEM). Eighteen New Zealand white rabbits were divided into three groups with 6 in each group. The model of sacrospinalis myobag was established by implanting above material samples in the myobag on the two sides. Every three rabbits in the three groups were killed at the 4^thand 8^th week after implantation, and then the new bone was observed by histologically (HE) and the areas of new osseous tissues were measured. Results from the inverted microscopy and ESEM showed that cells were grown well on the surface of the three materials, and BMP₂related peptide of Group A was confirmed to improve the adhesion rate and proliferation ability of MC3T3-E1 cells, and maintained the morphology of cells. At different postoperative time points, HE staining results showed new bone of Group A was significantly more than that of Group B and Group C, and that of Group B was significantly more than that of Group C. The areas of the new osseous tissue of group A, B, and C were (19 712.524±3 782.126) μm²,(28 227.617±2 455.375) μm², and (11 648.507±1 047.221) μm² at the 4^thweek postsurgery, and (14 592.892±899.532）μm², (7 986.655±903.487)μm², and (11254.822±669.508) μm² at 8^th week postsurgery respectively. Sintered bone with surface mineralization modification / P24 composite is an ideal scaffold for bone tissue engineering, which is expected to be applied to clinical studies.

Key words： surface modified sintered bone BMP₂-related peptide bone tissue engineering

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	LI Jing Feng^1*ZHENG Qi Xin² CHEN Liao Bin¹GUO Xiao Dong²ZOU Zhen Wei²

Cite this article:

LI Jing Feng^1*ZHENG Qi Xin² CHEN Liao Bin¹GUO Xiao Dong²ZOU Zhen Wei^{2. Tissue Engineered Bone Constructed by Sintered Bone with Surface Mineralization Modification/BMP-2-Related Peptide and MC3T3-E1 Cells[J]. journal1, 2014, 33(3): 343-348.}

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http://cjbme.csbme.org/EN/ 10.3969/j.issn.0258-8021. 2014. 03.012 OR http://cjbme.csbme.org/EN/Y2014/V33/I3/343

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