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| Study on Preparation and Properties of Immune Adsorbent for Rheumatoid Factorremoval in Hemoperfusion |
1 Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University,Chongqing 400044, China
2 Third Military Medical University, The First Affiliated Hospital, Integrated Traditional Chinese and Western Medicine, Department of Rheumatology Centre, Chongqing 400038, China |
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Abstract In this work we prepared an adsorbent (PS-PHE) aiming to remove rheumatoid factors and studied its performance. In the PSPHE, the chloromethyl resin acted as carrier grafting with the linker of heparin, to which phenylalanine was connected. The adsorption rate of adsorbent was investigated by in vitro RF dynamic perfusion. We conducted in vitro elution experiment to measure the abscission rate of RF. Selectivity of adsorbent was evaluated by blood components before and after perfusion. The prothrombin time (PTs) and thrombin time (TTs) detection in vitro verify the blood compatibility of adsorbent. The adsorption equilibrium was reached in less than two hours, and 90% of the maximum adsorption capacity was reached only under 1 hour, while the adsorption rate of IgA RF, IgG RF and IgM RF in serum was 45.21%±1.80%、56.02%±1.36%、52.40%±2.01% (n=5). After elution, the abscission rate of IgA RF, IgG RF and IgM RF was 22.10%±1.65%、19.23%±1.06%、21.31%±1.35% (n=5). After perfusion experiment, the variation rates of albumen and red blood cell were both less than 10%. The detection results of PTs and TTs showed PS-PHE delayed the coagulation rate. In conclusion, PS-PHE has higher absorption rate to RF and good blood compatibility, showing good application prospects.
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