Abstract The aim of this study is to investigate the effect of the composite liquid dressing in the treatment of acute skin ulcer for further applications in the prevention of surgical wound infection and promotion of wound healing. The composite liquid dressing (CLD) was prepared by carboxymethyl chitosan (CMC), polyvinyl butyral (PVB) and ethanol solution, according to the certain formulation. The performance and biological safety of CLD were evaluated by experiments of waterproof, breathable, bacteria resistance, and cell toxicity. Forty healthy adult SD rats, half male and half female, were made wound, carboxymethyl chitosan meanwhile different concentration at 1.0 mg/mL, 10.0 mg/mL, 30.0 mg/mLwas applied to the wound. The therapeutic effect of the wound composite liquid dressing was studied by daily observation and HE staining. The upper membrane liquid of CLD between 1.8～ 2.3 mm had very good waterproof permeability and resistance to bacteria. In animal models, the wound healing rate of the experimental group (10.0 and 30.0 mg/mL CMC) was 65.42% and 67.38%, which was higher than that of control group with significant difference (P< 0.01) on the 7th day. The wound healing rate of the experimental group (10.0 and 30.0 mg/mL CMC) was 100% on the 14th day. Seven days after the operations, the wound healing rate of the experimental group (10.0 and 30.0 mg/mL CMC), the stratified squamous epithelium that formed the epidermis and the dermis consisted of thin collagen fibers. The organization began to fall into the interior; the normal dermis with a thick, coarse collagen fiber was connected to a thin collagen fiber. The stratified squamous epithelium of the epidermis was much larger than that of the control group from 3 to 4. The wound was connected with dermal connective tissue. The skin was very close to the normal skin on the 12th day. The results showed that the dressing functioned (10.0 mg/mL CMC ) as a good barrier against the penetration of infection, it was waterproof, had a suitable water vapor transmission rate, good biosafety and a rapid wound closure rate.
Chen Yanpei,Xia Donglin,Wang Yufei, et al. The Fabrication of Composite Liquid Dressing and Applications in Skin Wound Healing[J]. Chinese Journal of Biomedical Engineering, 2016, 35(4): 453-459.
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